NPY基因多态性对番鸭产蛋性能的影响

doi:10.15933/j.cnki.1004-3268.2020.12.019

河南农业科学 ›› 2020, Vol. 49 ›› Issue (12): 130-136.DOI: 10.15933/j.cnki.1004-3268.2020.12.019

NPY基因多态性对番鸭产蛋性能的影响

周璇^1，2，3，谭斌^1，2，3，杨世皓^1，2，3，罗林丽^1，2，3，祝永才^1，2，3，王姣^1，2，3，杨胜林^1，2，3

（1.贵州大学高原山地动物遗传育种与繁殖教育部重点实验室，贵州贵阳 550025；2.贵州大学贵州省动物遗传育种与繁殖重点实验室，贵州贵阳 550025；3.贵州大学动物科学学院，贵州贵阳 550025）

收稿日期:2020-03-06 出版日期:2020-12-15 发布日期:2020-12-15
通讯作者: 杨胜林（1963-），男，贵州岑巩人，教授，博士，主要从事动物遗传育种与繁殖及特种经济动物饲养研究。E-mail:shenglinyang@126.com
作者简介:周璇（1996-），女，贵州开阳人，在读硕士研究生，研究方向：特种经济动物饲养。E-mail:15286056403@163com
基金资助:
贵州省科技厅成果应用与产业化计划项目［黔科合成果（2017）4110，（2018）4301］；贵州省科技厅科技支撑计划项目［黔科合NY（2016）2506、黔科合支撑（2017）2539、（2019）2283］；贵州省普通本科高等学校科技成果转化与产业化项目［黔教合KY字（2017）056］；贵州省工程中心建设项目［黔科合平台（2019）5203］

Effects of NPY Gene Polymorphism on Egg Production Performance of Muscovy Ducks

ZHOU Xuan^1,2,3,TAN Bin^1,2,3,YANG Shihao^1,2,3,LUO Linli^1,2,3,ZHU Yongcai^1,2,3,WANG Jiao^1,2,3,YANG Shenglin^1,2

(1.Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Rejion,Ministry of Education,Guizhou University,Guiyang 550025,China; 2.Key laboratory of Animal Genetics,Breeding and Reproduction of Guizhou Province,Guizhou University,Guiyang 550025,China; 3.College of Animal Science,Guizhou University,Guiyang 550025,China)

Received:2020-03-06 Published:2020-12-15 Online:2020-12-15

摘要/Abstract

摘要： 为探讨NPY基因变异对天柱番鸭产蛋性能的遗传效应，测定了番鸭产蛋性能指标，采用PCR产物纯化直接测序法筛查NPY基因单核苷酸多态性（SNP）位点，并分析其对产蛋性能的影响。结果表明，在番鸭NPY基因启动子区检测到2个中度多态的SNP位点（A411G和A540G），每个SNP位点均产生3种基因型（AA、AG和GG），其基因型分布均未偏离Hardy-Weinberg平衡（P>0.05）。共发现3种单倍型和4种双倍型。单倍型H1和双倍型H1H2频率最高，均为0.467。在开产日龄上，基因型AG极显著（P<0.01）早于基因型AA，双倍型H1H1显著（P<0.05）早于其他双倍型；在开产蛋质量上，基因型AG显著（P<0.05）高于基因型AA，双倍型H3H3显著（P<0.05）高于H1H1和H1H2；在300日龄产蛋量上，基因型GG极显著（P<0.01）高于基因型AA、基因型AG显著（P<0.05）高于基因型AA，双倍型H3H3显著（P<0.05）高于H1H2和H2H2。综上，基因型AG和双倍型H3H3的番鸭产蛋性能优异，这2个突变位点可作为产蛋性能选择的遗传标记。

关键词: 天柱番鸭, NPY基因, 基因多态性, SNP鉴定, 产蛋性能

Abstract: In order to investigate the genetic effect of NPY gene variation on egg production performance of Muscovy ducks,the egg production performance indicators of Muscovy ducks were determined.SNPs of NPY gene were screened by the method of directly sequencing of purified PCR products,and their effects on egg production performance were analyzed.The results showed that two moderate SNPs (A411G and A540G) which located on promoter region were detected in the NPY gene of Muscovy ducks.Each SNP produced three genotypes(AA、AG and GG),and the genotype distribution did not deviate from Hardy-Weinberg equilibrium(P>0.05).Three haplotypes and four diplotypes were found. Haplotype H1 and diplotype H1H2 displayed the highest frequency(both were 0.467).The laying start age of genotype AG was significantly(P<0.01) earlier than that of genotype AA,and diplotype H1H1 had a earlier laying start age compared to the other diplotype(P<0.05).Genotype GG had higher weight of the first egg compared to genotype AA(P<0.05),while diplotype H3H3 displayed higher weight of the first egg compared to the H1H1 and H1H2(P<0.05).For the 300-day egg yield,the genotype AG was significantly(P<0.01) higher than that of genotype AA,whereas the genotype GG was relatively higher than genotype AA(P<0.05),diplotype H3H3 had higher 300-day egg yield compared to H1H2 and H2H2(P<0.05).In summary,the genotype AG and the diplotype H3H3 Muscovy ducks displayed good egg production performance,these two SNPs could be used as genetic markers for egg production performance selection.

Key words: Muscovy ducks, NPY gene, Gene polymorphism, SNP identification, Egg-laying performance

中图分类号:

S834

周璇, 谭斌, 杨世皓, 罗林丽, 祝永才, 王姣, 杨胜林. NPY基因多态性对番鸭产蛋性能的影响[J]. 河南农业科学, 2020, 49(12): 130-136.

ZHOU Xuan, TAN Bin, YANG Shihao, LUO Linli, ZHU Yongcai, WANG Jiao, YANG Shenglin. Effects of NPY Gene Polymorphism on Egg Production Performance of Muscovy Ducks[J]. Journal of Henan Agricultural Sciences, 2020, 49(12): 130-136.

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NPY基因多态性对番鸭产蛋性能的影响

Effects of NPY Gene Polymorphism on Egg Production Performance of Muscovy Ducks

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