通草对泌乳期小鼠泌乳性能及泌乳相关内分泌激素的影响

doi:10.15933/j.cnki.1004-3268.2020.06.020

河南农业科学 ›› 2020, Vol. 49 ›› Issue (6): 150-156.DOI: 10.15933/j.cnki.1004-3268.2020.06.020

通草对泌乳期小鼠泌乳性能及泌乳相关内分泌激素的影响

刘莉莉¹，冯丽丽²，王博¹

（1.黑龙江中医药大学药学院，黑龙江哈尔滨 150040; 2.河南省农业科学院农业经济与信息研究所,河南郑州 450002）

收稿日期:2019-12-17 出版日期:2020-06-15 发布日期:2020-06-15
作者简介:刘莉莉（1980-），女，黑龙江铁力人，副教授，博士，主要从事中药对泌乳调控研究。E-mail：liulili-liulili@163.com
基金资助:
黑龙江省自然科学基金联合引导项目（LH2019H111）

Effect of Tetrapanax papyriferus on Lactation Performance and Endocrine Hormones Related to Lactation of Mice

LIU Lili¹,FENG Lili²,WANG Bo¹

(1.College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;2.Institute of Agricultural Economics and Information,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)

Received:2019-12-17 Published:2020-06-15 Online:2020-06-15

摘要/Abstract

摘要： 为了从内分泌系统角度探讨通草对泌乳动物乳腺泌乳的调节机制，采用高剂量12 g（生药）/mL和低剂量4 g（生药）/mL通草提取物灌服小鼠，检测其对泌乳期小鼠泌乳量的影响，应用ELISA法检测小鼠血清和乳腺组织匀浆液催乳素（PRL）、雌二醇（E₂）、孕酮（P）和生长激素（GH）的水平，并利用Western blot技术检测乳腺组织β-酪蛋白（β-casein）、催乳素受体（PRLR）、雌激素受体α（ERα）、雌激素受体β（ERβ）、孕酮受体（PR）、生长激素受体（GHR）蛋白的表达量。结果表明，与对照组（等量生理盐水）相比，高剂量和低剂量通草提取物均显著提高了泌乳期小鼠的泌乳量（P＜0.05），并明显促进小鼠乳腺β-酪蛋白的表达（P＜0.05）。通草提取物能显著提高泌乳期小鼠血清及乳腺组织PRL、E₂、GH的水平（P＜0.05）。与对照组相比，高剂量组和低剂量组小鼠血清PRL、E₂、GH的水平分别显著提高112.50%、129.15%、223.48%及49.84%、68.18%、100.76%。高剂量组和低剂量组小鼠乳腺PRL、E₂、GH的水平分别显著提高60.68%、269.35%、105.19%和32.27%、129.03%、53.68%。高剂量和低剂量通草提取物能明显提高乳腺组织PRLR、ERα、ERβ、GHR的表达量（P＜0.05），但对血清P及乳腺组织P、PR无显著性影响（P＞0.05）。可见，通草能通过提高小鼠泌乳相关内分泌激素水平及上调乳腺组织PRLR、ERα、ERβ和GHR的表达，进而促进小鼠乳腺泌乳。

关键词: 通草, 小鼠, 泌乳, 催乳素, 雌二醇, 孕酮, 生长激素

Abstract: In order to explore the regulation mechanism of Tetrapanax papyriferus on the lactation of mammary glands from the endocrine system,in this study,the mice were perfused with high dose 12 g(crude drug)/mL and low dose 4 g(crude drug)/mL of Tetrapanax papyriferus extracts.The effect of Tetrapanax papyriferus on milk yield of mice in lactation period was detected. The levels of PRL,E₂,P,GH in serum and mammary tissue homogenate of mice were detected by ELISA technology.The protein expression levels of β-casein,PRLR,ERα,ERβ,PR,GHR in mammary tissue were tested by Western blot technology.The results displayed that compared to the control group(the same volume of saline),high dose and low dose of Tetrapanax papyriferus extracts significantly increased the milk yield of mice in lactation period(P<0.05) and obviously promoted the expression of β-casein in mammary gland of mice(P<0.05).The levels of PRL,E₂,GH in serum and mammary tissue of mice were significantly enhanced by Tetrapanax papyriferus extracts (P<0.05).Compared with the control group,the levels of PRL,E₂,and GH in serum of mice in high dose group and low dose group respectively were significantly improved by 112.50%,129.15%,223.48% and 49.84%,68.18%,100.76%.The levels of PRL,E₂,and GH in mammary gland of mice in high dose group and low dose group respectively were obviously increased by 60.68%,269.35%,105.19% and 32.27%,129.03%,53.68%.The protein expression of PRLR,ERα,ERβ and GHR in mammary tissue were also evidently upregulated by high dose and low dose of Tetrapanax papyriferus extracts (P<0.05).However,P in serum and P,PR in mammary tissue were not significantly affected by Tetrapanax papyriferus extracts(P>0.05).This study shows that Tetrapanax papyriferus can promote the lactation of mammary gland by increasing the levels of endocrine hormones related to lactation and up regulating the expression of PRLR,ERα,ERβ and GHR in mammary gland.

Key words: Tetrapanax papyriferus, Mouse, Lactation, PRL, E₂, P, GH

中图分类号:

S859.3

刘莉莉, 冯丽丽, 王博. 通草对泌乳期小鼠泌乳性能及泌乳相关内分泌激素的影响[J]. 河南农业科学, 2020, 49(6): 150-156.

LIU Lili, FENG Lili, WANG Bo. Effect of Tetrapanax papyriferus on Lactation Performance and Endocrine Hormones Related to Lactation of Mice[J]. Journal of Henan Agricultural Sciences, 2020, 49(6): 150-156.

[1]	徐佳, 魏婉娥, 吴秋一, 释晓纯, 王烁, 陈兵. ENU 诱变构建Jag1基因mRNA 剪接异常眼畸形小鼠模型[J]. 河南农业科学, 2023, 52(9): 148-155.
[2]	赵园园, 孟金柱, 伍春亚, 潘春威. 牛卵巢黄体细胞增殖和孕酮分泌相关基因的筛选及其表达研究[J]. 河南农业科学, 2022, 51(10): 125-133.
[3]	田其真, 蔡丙严, 程汉, 郝福星, 黄银云. 野大黄散提取物的毒性分析[J]. 河南农业科学, 2020, 49(2): 159-164.
[4]	边艳杰，韩金汾，段江燕. miR-200b对奶牛乳腺上皮细胞泌乳功能的影响[J]. 河南农业科学, 2018, 47(7): 124-131.
[5]	付道斌，廖成水，路霞，王晓利，辛玲，肖宜航，宋香朋. 甘草多糖对小鼠及鼠伤寒沙门菌生长性能的影响[J]. 河南农业科学, 2018, 47(6): 139-143.
[6]	施玉华，王白石，孙红，季志鸿，王振龙. 左炔诺孕酮和炔雌醚对布氏田鼠繁殖的影响[J]. 河南农业科学, 2018, 47(3): 82-86.
[7]	朱雪雁，葛闻博，何玉琴,史军红，王文芳. 甘加藏羊发情周期不同阶段血浆生殖激素分泌规律[J]. 河南农业科学, 2017, 46(11): 138-143.
[8]	孙彦琴，任小丽，闫磊，闫跃飞，耿繁军，张震. 河南省奶牛生产性能测定数据质量分析[J]. 河南农业科学, 2016, 45(9): 140-145.
[9]	刘书伟，王燕，胡劲召. 槟榔鲜果对小鼠体质量、生精细胞凋亡及脏器系数的影响[J]. 河南农业科学, 2016, 45(2): 142-145,160.
[10]	刘书伟，王燕，都二霞，胡劲召. 石灰、蒌叶处理槟榔对小鼠生殖毒性及体温的影响[J]. 河南农业科学, 2016, 45(10): 151-154.
[11]	李英，徐志豪，张卓炜，谭志刚，胡莲美. 铜中毒对小鼠雄性生殖器官的影响[J]. 河南农业科学, 2015, 44(9): 122-124.
[12]	金鑫. 山药多糖对小鼠的抗应激作用研究[J]. 河南农业科学, 2015, 44(1): 143-145.
[13]	杨姗姗，张秀娟. 半枝莲多糖对荷瘤小鼠T细胞免疫功能的影响[J]. 河南农业科学, 2014, 43(6): 145-148.
[14]	宁维，陈利平，李瑜，李智奕，韩亚伟，郭亚鹏. 卷烟烟气体内安全评价方法研究[J]. 河南农业科学, 2013, 42(9): 152-156.
[15]	刘华，王晶，颜进项，罗强，张林西. 地黄多糖的超临界CO₂萃取及其对创伤小鼠脾脏的影响[J]. 河南农业科学, 2013, 42(6): 140-142，148.

通草对泌乳期小鼠泌乳性能及泌乳相关内分泌激素的影响

Effect of Tetrapanax papyriferus on Lactation Performance and Endocrine Hormones Related to Lactation of Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics