氯化汞对中国大鲵皮肤CATH-BF基因及心脏和胰腺CATH-2、CATH-3基因表达的影响

doi:10.15933/j.cnki.1004-3268.2020.01.021

河南农业科学 ›› 2020, Vol. 49 ›› Issue (1): 148-156.DOI: 10.15933/j.cnki.1004-3268.2020.01.021

氯化汞对中国大鲵皮肤CATH-BF基因及心脏和胰腺CATH-2、CATH-3基因表达的影响

叶婷，王兴香，刘亚男，曹宇，许抗抗，李灿

(贵阳学院生物与环境工程学院/贵州省山地珍稀动物与经济昆虫重点实验室，贵州贵阳 550005)

收稿日期:2019-07-09 出版日期:2020-01-15 发布日期:2020-01-15
通讯作者: 李灿(1977-)，男，贵州盘县人，教授，博士，主要从事有害生物控制与资源利用研究。E-mail:lican790108@163.com
作者简介:叶婷(1988-)，女，贵州盘县人，讲师，博士，主要从事环境毒理学研究。E-mail:tyerfhu@126.com
基金资助:
贵州省一流学科建设项目贵阳学院学科建设项目(黔教科研发［2017］85)；贵州省高层次创新人才“百”层次人才项目(黔科合人才［2016］4020号)；贵州省科学技术联合基金项目(黔科合LH字［2015］7311)；贵州省教育厅大鲵深加工技术开发产学研基地项目(黔教合KY字［2014］231)；贵州省大鲵可持续利用协同2011创新中心项目(黔教合协同创新字［2015］06)；贵州省科学技术基金项目(黔科合基础［2018］1005)；贵阳市科技局贵阳学院专项项目(GYU-KYZ［2018］02-11)

Effects of Mercuric Chloride on the Expression of CATH-BF in the Skin and CATH-2 and CATH-3 in the Heart and Pancreas of Chinese Giant Salamander(Andrias davidianus)

YE Ting,WANG Xingxiang,LIU Yanan,CAO Yu,XU Kangkang,LI Can

(College of Biology and Environmental Engineering,Guiyang University/Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region,Guiyang 550005,China)

Received:2019-07-09 Published:2020-01-15 Online:2020-01-15

摘要/Abstract

摘要： 为探讨氯化汞( HgCl₂)对大鲵皮肤CATH-BF基因及心脏和胰腺CATH-2、CATH-3基因表达的影响，将幼龄中国大鲵分别暴露于0 (对照)、1、10、100 ng/L HgCl₂ 中24、48、72 h，采用实时定量RT-PCR方法分析HgCl₂处理不同时间后，大鲵皮肤CATH-BF、心脏和胰腺中CATH-2、CATH-3基因的表达情况。结果显示，与对照组相比，大鲵暴露于HgCl₂ 24 h后，大鲵皮肤CATH-BF基因表达水平均呈现下降变化趋势，即1、100 ng/L HgCl₂对大鲵暴露24 h极显著下调了大鲵皮肤CATH-BF基因表达水平(P<0.01)，10 ng/L HgCl₂对大鲵暴露24 h显著下调了大鲵皮肤CATH-BF基因表达水平(P<0.05)，10、100 ng/L HgCl₂对大鲵暴露48 h显著诱导了大鲵皮肤CATH-BF基因表达(P<0.05)，大鲵暴露于HgCl₂ 72 h后，HgCl₂对大鲵皮肤CATH-BF基因表达无显著影响；大鲵暴露于HgCl₂ 24 h后，不同质量浓度HgCl₂对大鲵心脏CATH-2基因表达水平有不同的影响，即1、10 ng/L对大鲵暴露24 h极显著下调了心脏CATH-2基因表达水平(P<0.01)，100 ng/L HgCl₂对大鲵暴露24 h极显著上调了心脏CATH-2基因表达水平(P<0.01)，10、100 ng/L HgCl₂对大鲵暴露48 h后显著下调了大鲵心脏CATH-2基因表达水平(P<0.05)；10、100 ng/L HgCl₂对大鲵暴露24 h显著下调了大鲵胰腺CATH-2基因表达水平(P<0.05)；1、10 ng/L HgCl₂对大鲵暴露24 h极显著诱导了心脏CATH-3基因表达水平(P<0.01)，大鲵暴露于HgCl₂ 24、48、72 h后，HgCl₂ 对胰腺CATH-3基因表达均无显著性影响。可见，HgCl₂ 对大鲵皮肤CATH-BF基因及胰腺和心脏CATH-2、CATH-3基因表达的影响处于动态变化模式，并且HgCl₂对大鲵胰腺和心脏CATH-2、CATH-3基因表达影响存在组织特异性差异。

关键词: 中国大鲵, 氯化汞, CATH-BF基因, CATH-2基因, CATH-3基因

Abstract: In order to explore the effects of mercuric chloride(HgCl2) on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas of Chinese giant salamander(Andrias davidianus),the juvenile stage giant salamanders were continuously exposed to 0(control),1,10,and 100 ng/L HgCl₂ for 24,48,72 h respectively,and the effects of mercuric chloride on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas of Chinese giant salamander were investigated by real time quantitative RT-PCR method.After exposure to HgCl₂for 24 h,the expression level of CATH-BF in the skin of giant salamander was reduced compared with the control group.1 and 100 ng/L HgCl₂very significantly down-regulated the expression of CATH-BF in the skin (P<0.01). 10 ng/L HgCl₂ significantly down-regulated the expression of CATH-BF gene in the skin after exposure for 24 h(P<0.05).10 and 100 ng/L HgCl₂ significantly induced the expression of CATH-BF gene in the skin after exposure for 48 h(P<0.05).Exposure to HgCl₂ for 72 h had no significant effects on the expression of CATH-BF gene in the skin.Different concentrations of HgCl₂ had different effects on the expression of CATH-2 gene in the heart of the giant salamander.1 and 10 ng/L HgCl₂ significantly down-regulated the expression of CATH-2 gene in the heart after exposure for 24 h(P<0.01).100 ng/L HgCl₂ significantly up-regulated the expression of CATH-2 gene in the heart after exposure for 24 h(P<0.01).10 and 100 ng/L HgCl₂significantly down-regulated the expression of CATH-2 gene in the heart of the giant salamander after 48 h of exposure(P<0.05).10 and 100 ng/L HgCl₂ significantly down-regulated the expression of CATH-2 gene in the pancreas after 24 h of exposure(P<0.05).1 and 10 ng/L HgCl₂ significantly induced the expression of CATH-3 gene in the heart after exposure to HgCl₂ for 24 h(P<0.01).HgCl2 had no significant effect on the expression of CATH-3 gene in the pancreas after 24, 48 and 72 h of exposure.It was concluded that the pattern of effects of mercuric chloride on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas of Chinese giant salamander was dynamic,and the effects of HgCl₂ on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas showed tissue specificity.

Key words: Chinese giant salamander(Andrias davidianus), Mercuric chloride, CATH-BF gene, CATH-2 gene; CATH-3 gene

中图分类号:

S949

叶婷, 王兴香, 刘亚男, 曹宇, 许抗抗, 李灿. 氯化汞对中国大鲵皮肤CATH-BF基因及心脏和胰腺CATH-2、CATH-3基因表达的影响[J]. 河南农业科学, 2020, 49(1): 148-156.

YE Ting, WANG Xingxiang, LIU Yanan, CAO Yu, XU Kangkang, LI Can. Effects of Mercuric Chloride on the Expression of CATH-BF in the Skin and CATH-2 and CATH-3 in the Heart and Pancreas of Chinese Giant Salamander(Andrias davidianus)[J]. Journal of Henan Agricultural Sciences, 2020, 49(1): 148-156.

[1]	闫永峰;王兵丽. 煤矿塌陷区水污染对鱼类肝细胞DNA的损伤[J]. 河南农业科学, 2010, 39(4): 109-111.
[2]	郑晓军;郭二辉;胡小丽;吕丞;田朝阳. 河南忍冬属植物资源种类、生长型及生境研究[J]. 河南农业科学, 2009, 38(4): 102-104.
[3]	祁之秋;王英姿;夏天敏;李兴海;谷祖敏;程根武;纪明山. 拮抗细菌R26发酵滤液对灰葡萄孢菌的抑菌活性研究[J]. 河南农业科学, 2007, 36(12): 80-82.
[4]	何佳;陈钧;赵启美;祁红兵. 金钱松内生真菌抗植物病原真菌活性的研究[J]. 河南农业科学, 2007, 36(2): 46-49.

氯化汞对中国大鲵皮肤CATH-BF基因及心脏和胰腺CATH-2、CATH-3基因表达的影响

Effects of Mercuric Chloride on the Expression of CATH-BF in the Skin and CATH-2 and CATH-3 in the Heart and Pancreas of Chinese Giant Salamander(Andrias davidianus)

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics