河南农业科学 ›› 2020, Vol. 49 ›› Issue (1): 118-122.DOI: 10.15933/j.cnki.1004-3268.2020.01.016

• 园艺 • 上一篇    下一篇

60Co-γ射线对茉莉花插穗的辐照效应研究

李春牛,李先民,黄展文,卢家仕,卜朝阳
  

  1. (广西农业科学院花卉研究所,广西 南宁 530007)
  • 收稿日期:2019-08-19 出版日期:2020-01-15 发布日期:2020-01-15
  • 通讯作者: 卜朝阳(1966-),女,广西钦州人,研究员,主要从事生物技术研究。E-mail:yangnv@126.com
  • 作者简介:李春牛(1983-),男,湖南邵阳人,副研究员,硕士,主要从事花卉栽培与育种研究。E-mail:lichunniu@126.com
  • 基金资助:
    广西科技基地和人才专项(桂科AD17195065);广西重点研发计划项目(桂科AB18221064);广西农业科学院基本科研业务专项(2015YT91,2017YZ05); 南宁市技术创新引导专项(20182093)

Effects of 60Co-γ Irradiation on Cuttings of Jasminum sambac

LI Chunniu,LI Xianmin,HUANG Zhanwen,LU Jiashi,BU Zhaoyang   

  1. (Flower Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China)
  • Received:2019-08-19 Published:2020-01-15 Online:2020-01-15

摘要: 3个茉莉花品种插穗为材料,分别进行5个剂量的60Co-γ射线辐照处理,分析辐照剂量对插穗成活率、生根数量、最长根长及萌芽率的影响,通过辐照剂量与插穗相对成活率拟合回归直线,确定半致死剂量。结果表明,不同品种茉莉花对60Co-γ 射线辐照敏感性不同,5号茉莉花(多瓣茉莉)的半致死剂量为64.18 Gy,8号茉莉花(多瓣茉莉)为42.61 Gy,23号(双瓣茉莉)为39.57 Gy。与对照(0 Gy)相比,60Co-γ射线辐照显著降低插穗生根数量;30~60 Gy剂量的60Co-γ射线辐照显著抑制插穗萌芽率;40~60 Gy剂量辐照显著降低插穗的成活率、最长根长。

关键词: 茉莉花, 插穗; 60Co-γ射线, 半致死剂量

Abstract: In order to investigate the effect of 60 Co-γ irradiation on the survival rate,rooting number, longest root length and germination rate of Jasminum sambac cuttings,the cuttings from three cultivars of Jasminum sambac were respectively irradiated by five different irradiation doses.By fitting regression line between irradiation dose and relative survival rate of cuttings,we calculated the half lethal dose(LD50) of Jasminum sambac.The results showed that the survival rate varied significantly with different cultivars.The LD50 values of 60Co-γ irradiation for cuttings of No.5(double flowers),No.8(double flowers) and No.23(semi-double flowers) were respectively 64.18,42.61,39.57 Gy.The rooting number of cuttings decreased significantly with the increase of irradiation dose(from 0 Gy to 60 Gy).The germination rate of cuttings decreased significantly with the increase of irradiation dose(from 30 Gy to 60 Gy).The survival rate and longest root length of cuttings decreased significantly with the increase of irradiation dose(from 40 Gy to 60 Gy).

Key words: Jasminum sambac;Cuttings, 60Co-γ irradiation, Half lethal dose

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