四纹豆象Toll与抗菌肽基因对种群密度的响应

doi:10.15933/j.cnki.1004-3268.2019.09.013

河南农业科学 ›› 2019, Vol. 48 ›› Issue (9): 90-97.DOI: 10.15933/j.cnki.1004-3268.2019.09.013

四纹豆象Toll与抗菌肽基因对种群密度的响应

陈千权，王野影

（贵州师范大学生命科学学院，贵州贵安 550025）

收稿日期:2019-04-09 出版日期:2019-09-15 发布日期:2019-09-15
通讯作者: 王野影（1987-），女，黑龙江伊春人，讲师，博士，主要从事昆虫谱系地理学研究。E-mail:wangyeying0818@163.com
作者简介:陈千权（1983-），男，四川资阳人，讲师，博士，主要从事昆虫表观遗传学研究。E-mail:qqchen@gznu.edu.cn
基金资助:
贵州省科学技术厅-贵州师范大学联合基金项目（黔科合LH字［2017］7375号）；贵州师范大学博士启动基金项目（11904/0517070）

Ｒesponse of Expression Level of Tolls and Antimicrobial Peptides Genes to Population Density in Bean Beetle

CHEN Qianquan，WANG Yeying

(School of Life Sciences，Guizhou Normal University，Guian 550025，China)

Received:2019-04-09 Published:2019-09-15 Online:2019-09-15

摘要/Abstract

摘要： 为揭示四纹豆象（Callosobruchus maculatus）免疫信号转导与抗菌肽基因表达量对种群密度的响应机制，利用tBLASTn在四纹豆象腹部转录组数据中鉴定Toll、IMD（Immune deficiency）及抗菌肽基因，并以FPKM值指示基因表达量。结果表明，从腹部转录组中鉴定到6个富含亮氨酸结构域的Toll信号通路受体基因，其中，Toll-1进化速率较快，Toll-3、Toll-10、Toll-7、Toll-8、Toll-6进化速率依次降低。鉴定到IMD信号通路的胞内关键信号分子基因IMD和受体肽聚糖识别蛋白LC基因（Peptidoglycan recognition protein LC，PGRP-LC)。IMD和PGRP-LC的进化速率与Toll-3相似。表达量方面，Toll-1表达量最低，其FPKM值为0.32，且种群密度对其表达量无显著影响，Toll-3、Toll-6、Toll-7、Toll-8、Toll-10的表达量在高密度群体中显著或极显著上调，其中，Toll-7表达量最高，其在高密度群体、低密度群体中的FPKM值分别为127.25、57.92。种群密度对IMD和PGRP-LC的表达量无显著影响。鉴定到7个抗菌肽基因，即1个Defensin（防御素基因）、1个Cecropin（天蚕素基因）、2个Apidaecin（蜜蜂抗菌肽基因）、3个Drosomycin（抗真菌肽基因），其中，Drosomycin在进化方面最保守。Apidaecin-1表达量最低，其FPKM值为6.58，Defensin表达量最高，高密度群体、低密度群体的FPKM值分别为1 277.23、683.87。种群密度对Apidaecin-1、Cecropin、Apidaecin-2的表达量无显著影响，Defensin和3个Drosomycin在高密度群体中上调。综上所述，Toll-3、Toll-6、Toll-7、Toll-8、Toll-10与Defensin、Drosomycin-1、Drosomycin-2、Drosomycin-3在高种群密度条件下上调表达。

关键词: 四纹豆象；种群密度；免疫信号转导相关基因；抗菌肽； Toll, IMD

Abstract: In order to unveil the response mechanism of genes related to immunity signal transduction and antimicrobial peptide to population density，tBLASTn was used to identify genes related to Toll，IMD(Im mune deficiency) and antimicrobial peptide genes from ＲNA-sequencing data of bean beetle(Callosobru chus maculatus) .FPKM(Fragments per kilobase per million mapped reads) value was used to measure the expression level of these genes.The results showed that six Toll signaling pathway receptor genes which encoded proteins containing leucine-rich repeats domains were identified.Toll-1 had the fastest evo lutionary rate，while the evolutionary rates of Toll-3，Toll-10，Toll-7，Toll-8 and Toll-6 decreased in turn．IMD，an intracellular key signal gene，and Peptidoglycan recognition protein LC(PGＲP-LC) ，a receptor gene of IMD signaling pathway，were identified． IMD and PGＲP-LC had similar evolutionary rate to Toll 3.At expression level，Toll-1 was the lowest(FPKM=0.32) ，and was not regulated by population densi ty.The expression levels of Toll-3，Toll-6，Toll-7，Toll-8，and Toll-10 were significantly or extremely signif icantly up-regulated in high-density individuals，with Toll-7 having the highest expression level(its FP KMs in high-density individuals and low-density individuals were 127.25 and 57.92，respectively) ． Popu lation density had no significant effect on the expression level of IMD and PGＲP-LC.Seven antibacterial peptide genes were identified，including one Defensin(defensin gene) ，one Cecropin(cetanin gene) ，two Apidaecins(bee antibacterial peptide genes) ，and three Drosomycins(anti-fungal peptide genes) .Among them，Drosomycin was the most conservative one in evolution.Antibacterial peptide Apidaecin-1 had the lowest expression level(FPKM=6.58) and Defensin had the highest expression level(its FPKMs in high density individuals and low-density individuals were 1 277.23 and 683.87，respectively) .Population den sity had no significant effect on the expression level of Apidaecin-1，Cecropin and Apidaecin-2.Defensin and three Drosomycin genes were up-regulated in high density individuals.In summary，Toll-3，Toll-6， Toll-7，Toll-8，Toll-10，Defensin，Drosomycin-1，Drosomycin-2 and Drosomycin-3 were up-regulated under high population density．

中图分类号:

S433.4

陈千权, 王野影. 四纹豆象Toll与抗菌肽基因对种群密度的响应[J]. 河南农业科学, 2019, 48(9): 90-97.

CHEN Qianquan, WANG Yeying. Ｒesponse of Expression Level of Tolls and Antimicrobial Peptides Genes to Population Density in Bean Beetle[J]. Journal of Henan Agricultural Sciences, 2019, 48(9): 90-97.

参考文献

［1］WILSON K，THOMAS M B，BLANFORD S，et al.Coping with crowds: Densitydependent disease resistance in desert locusts［J］.Proceedings of the National Academy of Sciences，2002，99（8）：5471-5475.

［2］THEOPOLD U，SCHMIDT O，SODERHALL K，et al.Coagulation in arthropods: Defence，wound closure and healing［J］.Trends in Immunology，2004，25（6）：289-294.

［3］STRAND M R.The insect cellular immune response［J］.Insect Science，2008，15（1）：1-14.

［4］LEMAITRE B.The road to Toll［J］.Nat Rev Immunol，2004，4（7）：521-527.

［5］BETTENCOURT R,TANJI T，YAGI Y，et al.Toll and Toll9 in Drosophila innate immune response［J］.J Endotoxin Res，2004，10（4）：261-268.

［6］NAKAMOTO M，MOY R H，XU J，et al.Virus recognition by Toll7 activates antiviral autophagy in Drosophila［J］.Immunity，2012，36（4）：658-667.

［7］MOY R H，GOLD B，MOLLESTON J M，et al.Antiviral autophagy restricts rift valley fever virus infection and is conserved from flies to mammals［J］.Immunity，2014，40（1）：51-65.

［8］AKHOUAYRI I，TURC C，ROYET J，et al.Toll8/Tollo negatively regulates antimicrobial response in the Drosophila respiratory epithelium［J］.PLoS Pathogens，2011，7（10）：e1002319.

［9］YAGI Y，NISHIDA Y，IP Y T.Functional analysis of Toll related genes in Drosophila［J］.Development，Growth & Differentiation，2010，52（9）：771-783.

［10］BENTON M A，PECHMANN M，FREY N，et al.Toll genes have an ancestral role in axis elongation［J］.Current Biology，2016，26（12）：1609-1615.

［11］CHENG T C，ZHANG Y L，LIU C，et al.Identification and analysis of Toll related genes in the domesticated silkworm，Bombyx mori［J］.Developmental and Comparative Immunology，2008，32（5）：464-475.

［12］BINGSOHN L，KNORR E，BILLION A，et al.Knockdown of genes in the Toll pathway reveals new lethal RNA interference targets for insect pest control［J］.Insect Molecular Biology，2017，26（1）：92-102.

［13］MESSINA F J.Alternative lifehistories in Callosobruchus maculatus: Environmental and genetic bases［M］ //Bruchids and legumes: Economics，ecology and coevolution.Berlin:Springer，1990:303-315.

［14］SAYADI A，IMMONEN E，BAYRAM H，et al.The de novo transcriptome and its functional annotation in the seed beetle Callosobruchus maculatus［J］.PLoS One，2016，11（7）：e0158565.

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四纹豆象Toll与抗菌肽基因对种群密度的响应

Ｒesponse of Expression Level of Tolls and Antimicrobial Peptides Genes to Population Density in Bean Beetle

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