关键词: 蚯蚓, 中药渣, 发酵牛粪, 蛋白酶活性, 纤维素酶活性, 磷酸酶活性

Abstract: In order to solve the problem of environmental pollution caused by livestock and poultry feces and drug residues，and explore a new way of resource utilization of traditional Chinese medicine residues and cow dung，healthy earthworms with average body weight of 239.40 mg and average body length of 5.51 cm were selected and randomly divided into groups A，B，C and D，with three replicates in each group.On the basis of fermented cow dung，0，10%，20% and 30% pharmaceutical residues of Wuwei Jianpi granules were added，respectively，and earthworms were fed outdoors for 28 days.On the 0th and 28th days of feeding，9 earthworms were randomly selected from each group，and their body weight and body length were measured. At 0，7，14，21 and 28 days of feeding，9 earthworms were randomly selected from each group to extract crude enzyme solution，and enzyme linked immunosorbent assay was used to detect the activities of protease，cellulase and phosphatase.On the 28th day，compared with group A，the body weight and body length of earthworms in group C were significantly increased，the weight gain rate was increased by 55.50 percentage points，and the body length was increased by 1.83 cm.The activities of protease，cellulase and phosphatase in group C reached the highest（P<0.05）on the 28th day.The above results show that the drug residues of Wuwei Jianpi granules promote the growth and development of earthworms and enhance the digestive enzyme activities，and the best effect is obtained by adding 20% of the drug residues.

Key words: Earthworm, Traditional Chinese medicine residue, Fermented cow manure, Protease activity, Cellulase activity, Phosphatase activity