河南农业科学 ›› 2022, Vol. 51 ›› Issue (11): 42-49.DOI: 10.15933/j.cnki.1004-3268.2022.11.005

• 作物栽培·遗传育种 • 上一篇    下一篇

外源激素对干旱胁迫下油莎豆生长、产量及生理特性的影响

刘佳遥,程艳,魏尊苗,龙威,王靓,牟忠生   

  1. (吉林省农业科学院经济植物研究所,吉林 长春 130033)
  • 收稿日期:2022-06-13 出版日期:2022-11-15 发布日期:2023-01-09
  • 通讯作者: 牟忠生(1978-),男,吉林省长春人,副研究员,硕士,主要从事油莎豆栽培育种研究。E-mail:muzs@163.com
  • 作者简介:刘佳遥(1995-),男,黑龙江绥化人,研究实习员,硕士,主要从事油莎豆栽培育种研究。E-mail:jiayaoliu@yeah.net
  • 基金资助:
    国家重点研发计划项目(2019YFD1002603);吉林省重大科技专项(192480NY010166906);吉林省农业科技创新工程项目(CXGC2019DC007)

Effects of Exogenous Hormones on Growth,Yield and Physiological Characteristics of Tigernut(Cyperus esculentus L.)under Drought Stress

LIU Jiayao,CHENG Yan,WEI Zunmiao,LONG Wei,WANG Liang,MU Zhongsheng   

  1. (Economic Plant Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,China)
  • Received:2022-06-13 Published:2022-11-15 Online:2023-01-09

摘要: 为明确干旱胁迫下外源激素在油莎豆上的最佳喷施浓度,以吉莎2号为试验材料,采用15%聚乙二醇(PEG 6000)模拟干旱,研究不同浓度(质量浓度)赤霉素(GA3,0.1、0.2、0.3、0.4、0.5 μg/L)、褪黑素(MT,20、40、60、80、100 μmol/L)、水杨酸(SA,0.25、0.50、0.75、1.00、1.25 mmol/L)对油莎豆幼苗生长、产量及叶片相对电导率、叶绿素含量、脯氨酸含量、丙二醛含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性等生理生化指标的影响。结果表明,干旱胁迫下,喷施3种外源激素对油莎豆幼苗生长均具有促进作用,并提高产量,分别以G3(0.3 μg/L)、M3(60 μmol/L)、S2(0.50 mmol/L)处理效果最好,分别较不喷施激素的干旱胁迫处理(CK2)显著提高38.32%、31.73%、35.90%。干旱胁迫下,喷施3种外源激素总体上均能降低油莎豆幼苗叶片相对电导率和丙二醛含量,提高叶绿素含量、脯氨酸含量、SOD和POD活性,缓解干旱胁迫对油莎豆幼苗造成的伤害,分别以G3、M3、S2处理效果最好。其中,与CK2相比,G3、M3、S2处理相对电导率分别显著下降54.92%、34.44%、27.86%,叶绿素含量分别显著提高33.83%、19.03%、15.84%,脯氨酸含量分别显著提高478.88%、627.62%、412.87%,丙二醛含量分别显著下降53.51%、53.43%、54.75%,SOD活性分别提高0.95%、13.51%、16.25%,POD活性分别提高1.80%、10.36%、12.84%。综上,干旱胁迫下,喷施适宜浓度的GA3、MT、SA均能促进油莎豆生长,缓解干旱胁迫对油莎豆造成的伤害,进而提高产量,总体上分别以0.3 μg/L、60 μmol/L、0.50 mmol/L处理效果最好。

关键词: 油莎豆, 外源激素, 干旱胁迫, 幼苗生长, 生理特性

Abstract: In order to determine the optimal spraying concentration of exogenous hormones on tigernut under drought stress,Jisha No.2 was used as material,drought stress was simulated by 15% polyethylene glycol(PEG 6000)to study the effects of different concentrations of gibberellin(GA3,0.1,0.2,0.3,0.4,0.5 μg/L),melatonin(MT,20,40,60,80,100 μmol/L)and salicylic acid(SA,0.25,0.50,0.75,1.00,1.25 mmol/L) on the growth,yield,relative conductivity,chlorophyll content,proline content,malondialdehyde(MDA)content,superoxide dismutase(SOD)and peroxidase(POD)activities of tigernut seedlings.The results showed that under drought stress,spraying the three kinds of exogenous hormones could promote the growth and yield of tigernut,G3(0.3 μg/L),M3(60 μmol/L),S2(0.50 mmol/L)treatments had the best effect,the yields of which significantly increased by 38. 32%,31.73% and35.90% compared with the drought stress treatment(CK2)respectively. Under drought stress,spraying the three kinds of exogenous hormones could reduce the relative conductivity and MDA content in leaves,and increase the chlorophyll content,proline content,SOD and POD activities,alleviating the damage caused by drought stress to the seedlings of tigernut,the best treatments were G3,M3 and S2,respectively.Compared with CK2,for G3,M3 and S2 treatments,the relative conductivities significantly decreased by 54.92%,34.44% and 27.86%,chlorophyll contents obviously increased by 33.83%,19.03% and 15.84%,proline contents significantly increased by 478.88%,627.62% and 412.87%,MDA contents significantly decreased by 53.51%,53.43% and 54.75%,SOD activities increased by 0.95%,13.51%and 16.25%,POD activities increased by 1.80%,10.36% and 12.84%,respectively.In conclusion,under drought stress,spraying appropriate concentrations of GA3,MT and SA could promote the growth of tigernut,alleviate the damage caused by drought stress,and then increase the yield,overall the best concentrations of GA3,MT and SA were 0.3 μg/L,60 μmol/L and 0.50 mmol/L,respectively.

Key words: Tigernut(Cyperus esculentus L.), Exogenous hormones, Drought stress, Seedling growth, Physiological characteristics

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