In order to explore the structure and function of the protein encoded by the solute carrier 13(SLC13) gene family of bovine,this study used bioinformatics softwares to analyze the biological property(including the physicochemical properties,hydrophobicity,secondary structure,tertiary structure,subcellular localization and transmembrane region of the protein), predict the protein interaction networks and construct phylogenetic tree of the SLC13 gene family-encoded proteins of different species.The results showed that the number of amino acids encoded by the SLC13 gene family of bovine was from 520 to 626,the highest content was leucine and the lowest was histidine.The molecular mass ranged from 57.80 ku to 68.96 ku.All proteins were hydrophobic protein and all proteins except for SLC13A2 were stable.The secondary structure of the SLC13 protein gene was mainly composed of α-helix,β-turn and random coil,and the number of exons was from 11 to 16.Except that the SLC13A3 protein gene did not have Motif 10and the SLC13A5 protein did not have Motif 8, all proteins had Motif 1—10 in the sequence.The subcellular localization of SLC13 protein was in the plasma membrane, endoplasmic reticulum and mitochondria,and a small amount of SLC13A3 protein was found in the Golgi apparatus.The SLC13 protein was the inner membrane protein of multiple transmembrane helix,and it played a role in different forms at the biological level and molecular function level. Phylogenetic analysis found that the SLC13A1 gene of 14 species was in the same larger branch as the SLC13A4 gene,and SLC13A2,SLC13A3 and SLC13A5 were in another large branch.The common bovine had the closest relationship with zebu,buffalo and yak.