In order to explore the utilization value of Litsea cubeba residue(LCR) in ruminant feed,Leymus chinensis(LC,control) was selected as the control,and rumen fluid of Hu sheep for simulating rumen fermentation cultivation in vitro was collected,which fermented for 48 h with LCR to study the effects of rumen fermentation by gas production technique. The results showed as followed:(1) The contents of dry matter(DM),crude protein(CP),ether extract(EE),neutral detergent fiber(NDF) and acid detergent fiber(ADF) of LCR were 95.18%,13.01%,34.74%,44.06% and 11.08%(DM basis),respectively.(2) After 48 hours of gas production in vitro,the total gas production of LCR was -1.53 mL/g,which was extremely significantly(P<0.01) lower than that of LC.The pH of the fermentation broth of LCR was 6.67,which was significantly(P<0.05) higher than LC(6.54).The concentration of total volatile fatty acids(TVFA) in the fermentation broth of LCR was 8. 74 mmol/L,which was extremely significantly(P<0.01) lower than LC(39.85 mmol/L).Compared with LC group,the levels of acetic acid,propionic acid,butyric acid,isobutyric acid and isovaleric acid were all extremely significantly(P<0.01) lower,and the levels of valeric acid was significantly(P<0.05) lower in the fermentation broth of LCR,while there was no significant(P>0.05) difference in the ratio of acetic acid and propionic acid between groups.In conclusion,LCR is a kind of feed resources with rich nutritional value,but it has an inhibitory effect on the growth of rumen microorganisms,which is bad for rumen fermentation and gas production in ruminants.