Agricultural Resources and Environment

 Determination of Herbicide Atrazine Degradation Products by Strain Enterobacter sp.and Degradation Gene Cloning

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  •  (Department of Environmental and Safety Engineering,Shenyang University of Chemical Technology,Shenyang 110142,China)

Received date: 2019-05-21

  Online published: 2019-10-15

Abstract

 In order to analyze the biodegradation process of herbicide atrazine,liquid chromatography mass spectrometry was used to detect the degradation products of atrazine by functional strain Enterobacter sp.The degradation gene was cloned by the PCR amplification technology,and the degradation gene func tion was detected by using the GO function enrichment analysis.Hydroxyatrazine,cyanuric acid and urea were successively detected in the degradation products,and the degradation genes L465-RS09425,L465 RS10465,L465-RS12445,L465-RS09100,L465-RS12200 and UreA were cloned.It was speculated that strain Enterobacter sp.could mineralize atrazine gradually by dechlorination,dealkylation,deamination,ring cleavage,decarboxylation and hydrolysis of allophanate under the action of degrading genes.

Cite this article

LIU Dandan, SUN Wanyu, LIU Chang, MA Haoke, WANG Ruijiao, SUI Yufan .  Determination of Herbicide Atrazine Degradation Products by Strain Enterobacter sp.and Degradation Gene Cloning[J]. Journal of Henan Agricultural Sciences, 2019 , 48(10) : 70 -76 . DOI: 10.15933/j.cnki.1004-3268.2019.10.011

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