Genetic Diversity and Population Structure of Angelica dahurica Germplasm Resources

doi:10.15933/j.cnki.1004-3268.2025.07.007

Abstract

Abstract: To clarify the genetic diversity and population structure characteristics of Angelica dahurica parent materials and improve the utilization efficiency of A.dahurica germplasm resources，28 start codon targeted polymorphism（SCoT）markers were used for PCR amplification of 78 germplasm accessions from 5 A.dahurica populations.Genetic diversity and population structure were analyzed using softwares such as POPGENE 1.32 and GenALEx6.502.The results showed that 28 SCoT markers amplified a total of 192 bands，including 159 polymorphic bands.The average number of amplified bands and polymorphic bands per marker were 7 and 6，respectively.The average values of polymorphic information content（PIC），Shannon’s diversity index（I），and Nei’s gene diversity index（H）for SCoT markers were 0.800，0.350，and 0.240，respectively，indicating relatively high genetic diversity. For the five A.dahurica populations，the average number of alleles per locus（N_a），average effective number of alleles（N_e），average I，and average expected heterozygosity（H_e）were 1.514，1.293，0.265，and 0.174，respectively，with the Yu A.dahurica population showing the highest values.The genetic differentiation coefficient and gene flow among the five populations were 0.135 and 3.195，respectively.94% of the genetic variation originated within populations，and high gene flow reduced genetic differences between populations.Based on genetic similarity coefficients（GS）among populations，the five A.dahurica populations were clustered into three groups at a GS threshold of 0.96：Yu A.dahurica，Hang A.dahurica，and Chuan A.dahurica populations were grouped together.Based on the GS of 78 A.dahurica germplasms，they were clustered into three major categories at a GS value of 0.765.These categories included 73，3，and 2 A.dahurica germplasms respectively. In conclusion，the five A.dahurica populations exhibit low levels of genetic diversity and close genetic relationships among germplasms.Therefore，efforts should be strengthened to create new A.dahurica germplasm resources.

Key words: Angelica dahurica, Germplasm, SCoT marker, Genetic diversity, Population structure, Cluster analysis

摘要： 为探明白芷亲本中材料的遗传多样性和群体结构特点，提高白芷种质的利用效率，选用28个目标起始密码子多态性（Start codon targeted polymorphism，SCoT）标记对来自5个白芷居群的78份种质进行PCR扩增，采用POPGENE 1.32、GenALEx6.502等软件研究其遗传多样性和群体结构。结果表明，28个SCoT标记共扩增出192个条带，其中多态性条带159个，平均每个标记的扩增条带数和多态性条带数分别是7、6个；SCoT标记的平均多态性信息含量值（PIC）、平均Shannon’s多样性指数（I）和平均Nei’s基因多样性指数（H）分别为0.800、0.350、0.240，表现出较高的遗传多样性。5个白芷居群每个位点平均等位基因数（N_a）、平均有效等位基因数（N_e）、平均I 和平均期望杂合度（H_e）分别为1.514、1.293、0.265、0.174，以禹白芷居群的N_a、N_e、I、H_e最高。5个白芷居群遗传分化系数和基因流分别为0.135、3.195，94%遗传变异来自居群内，较大的基因流减少了居群间遗传差异。基于居群间的遗传相似系数（GS）在0.96处将5个白芷居群聚为三大类，其中禹白芷、杭白芷和川白芷居群被聚为一类；基于白芷种质的GS在0.765处将其聚为三大类，分别包含73、3、2份白芷种质。综上，5个白芷居群遗传多样性水平较低，种质间的亲缘关系较近，应加强白芷种质资源创制工作。

关键词: 白芷, 种质, SCoT标记, 遗传多样性, 群体结构, 聚类分析

CLC Number:

XU Lanjie, AN Sufang, YU Yongliang, DONG Wei, LIANG Huizhen, TAN Zhengwei, YANG Qing, YANG Hongqi, WU Xiaohui. Genetic Diversity and Population Structure of Angelica dahurica Germplasm Resources[J]. Journal of Henan Agricultural Sciences, 2025, 54(7): 64-71.

许兰杰, 安素妨, 余永亮, 董薇, 梁慧珍, 谭政委, 杨青, 杨红旗, 吴晓慧. 白芷种质遗传多样性与群体结构分析[J]. 河南农业科学, 2025, 54(7): 64-71.

Figures/Tables 6

References

［1］王梦月，贾敏如. 白芷本草考证［J］. 中药材，2004，27 （5）：382‑385.

WANG M Y，JIA M R. The herbalogical textual research on “Bai Zhi”［J］. Journal of Chinese Medicinal Materials，2004，27（5）：382‑385.

［2］徐方飚，王新志，孙田烨，等. 基于网络药理学白芷香豆素类镇痛作用研究［J］. 中华中医药学刊，2021，39（3）：196‑199.

XU F B，WANG X Z，SUN T Y，et al. Preliminary study on analgesic effect of coumarin in Baizhi （Angelicae dahuricae Radix）based on network pharmacology［J］.Chinese Archives of Traditional Chinese Medicine，2021，39（3）：196‑199.

［3］郭四红.白芷痤康散外敷治疗痤疮47例疗效观察［J］.河南职工医学院学报，2005，17（1）：41‑42.

GUO S H. Acne treated by baizhicuokang［J］. Journal of Henan Medical College for Staff and Workers，2005，17 （1）：41‑42.

［4］李海馨，何婧芝，冯文亚，等. 白芷美白活性部位初步研究［J］.天然产物研究与开发，2018，30（3）：425‑428.

LI H X，HE J Z，FENG W Y，et al. Preliminary studies on the active fraction responsible for whitening activity of Angelicae dahuricae［J］. Natural Product Research and Development，2018，30（3）：425‑428.

［5］周熠，那立欣. 白芷药食同源研究进展［J］. 亚太传统医药，2022，18（3）：213‑217.

ZHOU Y，NA L X. Research progresson of medicinal and edible plant Angelica dahurica［J］. Asia‑Pacific Traditional Medicine，2022，18（3）：213‑217.

［6］邹菊英，苏维，潘意，等. 白芷化学成分和药理作用研究进展及其质量标志物（Q-marker）的预测分析［J］.世界科学技术-中医药现代化，2023，25（7）：2535‑2548.

ZOU J Y，SU W，PAN Y，et al. Chemical components and pharmacological action for Angelica dahurica sinensis and predictive analysis on its Q‑marker［J］.Modernization of Traditional Chinese Medicine and Materia Medica‑World Science and Technology，2023，25 （7）：2535‑2548.

［7］国家药典委员会. 中华人民共和国药典（一部）［S］. 北京：中国医药科技出版社，2020：91.

Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China（Part 1）［S］.Beijing：China Medical Science and Technology Press，2020：91.

［8］吉庆，马宇衡，张烨. 白芷的化学成分及药理作用研究进展［J］.食品与药品，2020，22（6）：509‑514.

JI Q，MA Y H，ZHANG Y. Research progress on chemical constituents and pharmacological effects of Angelicae dahuricae Radix［J］. Food and Drug，2020，22 （6）：509‑514.

［9］赵东岳，郝庆秀，金艳，等. 白芷生物学特性及栽培技术研究进展［J］. 中国现代中药，2015，17（11）：1188‑1192.

ZHAO D Y，HAO Q X，JIN Y，et al. Research advance on the biological characteristics and cultivation technologies of Angelica dahurica［J］. Modern Chinese Medicine，2015，17（11）：1188‑1192.

［10］COLLARD B C Y，MACKILL D J. Start codon targeted（SCoT）polymorphism：A simple，novel DNA marker technique for generating gene‑targeted markers in plants［J］. Plant Molecular Biology Reporter，2009，27（1）：86‑93.

［11］许兰杰，梁慧珍，余永亮，等. 红花农艺性状与SCoT标记的关联分析［J］.江苏农业科学，2024，52（4）：157‑162.

XU L J，LIANG H Z，YU Y L，et al. Association analysis between SCoT markers and agronomic traits of safflower［J］. Jiangsu Agricultural Sciences，2024，52（4）：157‑162.
［12］王令，李佼，席彦军，等. 基于SCoT标记的陕西茶树种质资源遗传多样性分析［J］.西北农业学报，2018，27（2）：244‑252.
WANG L，LI J，XI Y J，et al. Analysis on genetic diversity of tea germplasm in Shaanxi based on SCoT markers［J］. Acta Agriculturae Boreali‑Occidentalis Sinica，2018，27（2）：244‑252.

［13］许兰杰，杨青，余永亮，等. 不同居群艾种质遗传多样性和遗传结构分析［J］.北方园艺，2024（2）：94‑100.

XU L J，YANG Q，YU Y L，et al. Genetic diversity and population structure of different populations in Artemisia argyi germplasm［J］. Northern Horticulture，2024（2）：94‑100.

［14］沈浩，刘登义. 遗传多样性概述［J］. 生物学杂志，2001，18（3）：5‑7.

SHEN H，LIU D Y. Summary of genetic diversity［J］.Journal of Biology，2001，18（3）：5‑7.

［15］陶小所，姚晓华，吴昆仑，等. 基于表型和基因型的藜麦种质资源遗传多样性分析［J］. 西北农业学报，2024，33（5）：798‑809.

TAO X S，YAO X H，WU K L，et al. Genetic diversity analysis of quinoa germplasm resources based on phenotype and genotype［J］.Acta Agriculturae Boreali‑Occidentalis Sinica，2024，33（5）：798‑809.

［16］刘粉粉，莫亮团，欧国腾，等.中国野生刺梨的遗传多样性及遗传结构分析［J］.植物科学学报，2024，42（3）：350‑358.

LIU F F，MO L T，OU G T，et al.Genetic diversity and genetic structure analysis of wild Chinese Rosa roxburghii Tratt.germplasm resources［J］.Plant Science Journal，2024，42（3）：350‑358.

［17］翟冰欣，黄春燕，王宇，等.68份甜荞种质资源产量性状遗传多样性分析及综合评价［J］.南方农业学报，2025，56（4）：1045‑1055.

ZHAI B X，HUANG C Y，WANG Y，et al. Genetic diversity analysis and comprehensive evaluation of yield traits of 68 Fagopyrum esculentum germplasm resources transcriptome analysis of taro（Colocasia esculenta）leaves under drought stress［J］. Journal of Southern Agriculture，2025，56（4）：1045‑1055.

［18］车雅丽，吴苏峻，王坤杨，等. 镉胁迫下野生大豆苗期性状全基因组关联分析及耐镉候选基因筛选［J］.河南农业科学，2025，54（1）：40‑54.

CHE Y L，WU S J，WANG K Y，et al. Genome‑wide association analysis of wild soybean seedling traits and screening of candidate cadmium‑tolerant genes［J］.Journal of Henan Agricultural Sciences，2025，54（1）：40‑54.

［19］田慧鑫，于婵，杨帆，等. 河南省牛至及其近缘种甘牛至SRAP遗传多样性分析与指纹图谱构建［J］.河南农业科学，2025，54（1）：55‑65.

TIAN H X，YU C，YANG F，et al. Genetic diversity analysis and fingerprint construction of Henan Origanum vulgare and its related species Origanum majorana by SRAP markers［J］. Journal of Henan Agricultural Sciences，2025，54（1）：55‑65.

［20］张晓磊，王晓明，范光宇，等. 基于主成分分析和聚类分析的谷子种质资源主要农艺性状综合评价［J］.山西农业科学，2025，53（1）：1‑11.
ZHANG X L，WANG X M，FAN G Y，et al.Comprehensive evaluation of main agronomic traits of millet germplasm resources based on principal component analysis and cluster analysis［J］. Journal of Shanxi Agricultural Sciences，2025，53（1）：1‑11.

［21］叶建强，张芳芳，陈娜，等. 金顶侧耳种质资源遗传多样性分析及耐高温种质筛选［J］.南方农业学报，2024，55（12）：3517‑3532.
YE J Q，ZHANG F F，CHEN N，et al. Genetic diversity analysis and high temperature resistance screening of Pleurotus citrinopileatus germplasm resources［J］.Journal of Southern Agriculture，2024，55（12）：3517‑3532.

［22］吴民华，吴子健，叶晓霞，等. 鸡矢藤叶绿体基因组分析［J］.河南农业科学，2024，53（1）：70‑77.
WU M H，WU Z J，YE X X，et al. Analysis on chloroplast genome of Paederia scandens［J］. Journal of Henan Agricultural Sciences，2024，53（1）：70‑77.

［23］桂腾琴，张思平，武忠亮，等. 白及不同地理群体的分子遗传学评价［J］. 北方园艺，2024（10）：105‑111.
GUI T Q，ZHANG S P，WU Z L，et al. Molecular genetic evaluation of different geographical populations of Bletilla striata Rchb. f.［J］. Northern Horticulture，2024（10）：105‑111.

［24］张晓煜，王仕鹏，曹昆山，等. 基于表型性状与SSR标记的马铃薯种质资源遗传多样性研究［J］.西北农业学报，2024，33（8）：1436‑1447.
ZHANG X Y，WANG S P，CAO K S，et al. Genetic diversity of potato germplasm resources based on phenotypic traits and SSR markers ［J］. Acta Agriculturae Boreali‑Occidentalis Sinica，2024，33（8）：1436‑1447.

［25］郭丁丁，马逾英，唐琳，等. 白芷种质资源遗传多样性的ISSR研究［J］.中草药，2009，40（10）：1627‑1630.
GUO D D，MA Y Y，TANG L，et al. Genetic diversity of Radix Angelicae dahuricae germplasmic resource based on ISSR analysis［J］.Chinese Traditional and Herbal Drugs，2009，40（10）：1627‑1630.
［26］侯凯，吴卫，郑有良，等. 川产白芷遗传多样性的ISSR分析［J］.四川农业大学学报，2008，26（3）：237‑240.

HOU K，WU W，ZHENG Y L，et al. Genetic diversity of Angelicae dahuricae accessions from Sichuan Province by inter‑simple sequence repeat（ISSR）［J］.Journal of Sichuan Agricultural University，2008，26（3）：237‑240.
［27］马逾英，熊英，贾敏如，等. 川白芷种质的RAPD遗传标记研究［J］.世界科学技术-中医药现代化，2004，6（2）：76‑79.

MA Y Y，XIONG Y，JIA M R，et al. Study on RAPD genetic markers of germenplasm of Radix Angelicae dahuricae［J］. Modernization of Traditional Chinese Medicine and Materia Medica‑World Science and Technology，2004，6（2）：76‑79.
［28］黄璐琦，王敏，付桂芳，等. 中药白芷种质资源的RAPD分析［J］. 中国中药杂志，1999，24（8）：457‑459.
HUANG L Q，WANG M，FU G F，et al.RAPD analysis of germplasm in "Baizhi［J］.China Journal of Chinese Materia Medica，1999，24（8）：457‑459.
［29］沈晓霞，沈宇峰，江建铭. 杭白芷种质资源遗传多样性的AFLP 分析［J］.浙江农业学报，2012，24（6）：1021‑1025.
SHEN X X，SHEN Y F，JIANG J M. AFLP analysis of genetic diversity of Angelica dahurica［J］. Acta Agriculturae Zhejiangensis，2012，24（6）：1021‑1025.

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