Journal of Henan Agricultural Sciences ›› 2023, Vol. 52 ›› Issue (11): 133-145.DOI: 10.15933/j.cnki.1004-3268.2023.11.015

• Animal Husbandry and Veterinary Medicine • Previous Articles     Next Articles

Comparative Analysis of the Microbial Community Structure in Litopenaeus vannamei Intestine and Surrounding Environment from Different Culture Patterns

HOU Qiang1,ZHOU Xin1,ZHANG Jianxiong1,MA Ruixin2,WU Jiang’ai1,CUI Ya’nan1,CHEN Yu1,ZHENG Baiqin1,ZHANG Lifang1   

  1. (1.Tangshan Food and Drug Comprehensive Testing Center/Key Laboratory of Quality Evaluation and Nutrition Health of Agro⁃Products,Ministry of Agriculture and Rural Affairs,Tangshan 063000,China;2.Hebei Fisheries Technology Extension Center,Shijiazhang 050000,China)
  • Received:2023-04-06 Published:2023-11-15 Online:2023-12-05

不同养殖模式下凡纳滨对虾肠道及环境微生物群落结构分析与比较

侯蔷1,周鑫1,张建雄1,马瑞欣2,吴江爱1,崔亚楠1,陈宇1,郑百芹1,张丽芳1   

  1. (1.唐山市食品药品综合检验检测中心/农业农村部农产品品质评价与营养健康重点实验室,河北 唐山063000;2.河北省水产技术推广总站,河北 石家庄 050000)
  • 通讯作者: 张丽芳(1982-),女,河北唐山人,高级兽医师,硕士,主要从事农产品质量安全检测研究。E-mail:928747109@qq.com
  • 作者简介:侯蔷(1987-),女,河北唐山人,高级畜牧师,博士,主要从事水产健康养殖与质量安全检测研究。E-mail:houqiang1130@sina.com
  • 基金资助:
    河北省重点研发计划项目(20327505D);河北省高端人才项目

Abstract: The present study aimed to explore the microbial community structure in Litopenaeus vannamei intestine and surrounding environment of different culture patterns in Tangshan area of Heibei Province,and to contrast the variations between them.16S rRNA high⁃throughput sequencing technology combined with bioinformatics methods was used to analyze the microbial diversity,community structure composition and variations in L.vannamei intestine,surrounding water and sediment from different culture patterns including freshwater intensive culture(FI)and freshwater mixed culture(FM).The results showed that sequences of FI and FM patterns were clustered into 6 077 and 7 961 operational taxonomic units(OTUs)respectively.Sediment samples had significantly higher microbial diversity than water and shrimp intestine samples from both patterns.Compared with FI pattern,diversity in sediment samples of FM pattern was higher.Proteobacteria and Bacteroidetes were the most prevalent phyla across all sample types,while all types of samples varied in terms of the dominating genus and its relative abundance.Compared to shrimp intestine and water samples,the microbial species number and richness in the sediment samples were the highest.Cluster analysis showed that the composition of the microbial communities in shrimp intestines resembled sediment samples more than water samples.The research showed that the number of microbial species in L.vannamei intestine and surrounding environment from two kinds of freshwater culture patterns in Tangshan remained constant while the relative abundance and diversity of the microorganisms rose with time.In comparison to the FI pattern,the FM pattern contained a greater number of microbial species in each type of samples,and the relative abundance of species,especially Vibrio,changed more significantly at the final stage of culture.

Key words: Litopenaeus vannamei, Microbial community structure, Intensive culture, Mixed culture, High?throughput sequencing

摘要: 为探究河北省唐山市不同养殖模式下凡纳滨对虾肠道及养殖环境微生物群落结构及差异,采用16S rRNA高通量测序技术和生物信息学方法,分析了淡水精养(Freshwater intensive culture,FI)和混养(Freshwater mixed culture,FM)2种养殖模式不同时期对虾肠道、水体及底泥微生物的多样性、物种组成及差异。结果表明,FI模式所有样品共聚类为6 077个可操作分类单元(OTU),FM模式所有样品共聚类为7 961个OTU。2种模式的底泥微生物多样性均显著高于水体和对虾肠道,且FM模式底泥的多样性更高。在门水平上,变形菌门(Proteobacteria)是所有样品中相对丰度均较高的物种,其次为拟杆菌门(Bacteroidetes);在属水平上,不同养殖模式下不同样品的优势菌属及相对丰度各不相同。相较于对虾肠道和水体,底泥中微生物的数量和丰富度均为最高;底泥与对虾肠道微生物种类及组成较为相似。研究表明,唐山市2种凡纳滨对虾淡水养殖模式下对虾肠道与环境微生物物种数量随养殖时间的变化基本不变,但物种群落丰富度及多样性均有一定提高;相较于FI模式,FM模式各类型样品微生物群落物种数量更多,且养殖末期时物种相对丰度的变化更加显著,特别是对虾肠道中弧菌属细菌相对丰度显著提高。

关键词: 凡纳滨对虾, 微生物群落结构, 精养, 混养, 高通量测序

CLC Number: