doi:10.15933/j.cnki.1004-3268.2018.03.007

Journal of Henan Agricultural Sciences ›› 2018, Vol. 47 ›› Issue (3): 34-37.DOI: 10.15933/j.cnki.1004-3268.2018.03.007

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Received:2017-12-22 Published:2018-03-15 Online:2018-03-15

郑麦9023胚性悬浮细胞系的建立和植株再生

李冬兵，吕桂珍，牛洪斌，尹钧

1 河南农业大学，河南粮食作物协同创新中心/小麦玉米作物学国家重点实验室/国家小麦工程技术研究中心，河南郑州 450002 2 华南师范大学生命科学学院，广东省高校生态学与环境科学重点实验室，广东广州 510631

通讯作者: 尹钧（1957-），男，山西万荣人，教授，博士，研究方向为小麦生育生态、小麦高产栽培生物技术。E-mail：xmzxyj@126.com
作者简介:李冬兵（1986-），男，湖北随州人，博士，研究方向为作物生物技术。E-mail：ldb06138233@163.com
基金资助:
“十二五”国家科技支撑计划重大项目（2011BAD16B07）

Abstract

摘要： 为建立高效稳定的小麦遗传转化体系，以小麦郑麦9023的幼胚为外植体诱导愈伤组织，研究了培养基中不同质量浓度2，4-D、KT对愈伤组织诱导和愈伤组织继代培养的影响，进而完成整个植株再生的进程。结果表明：以小麦郑麦9023的幼胚作为外植体来诱导愈伤组织，诱导效果最好的培养基是含有 3.0mg/L 2，4-D的MS培养基，出愈率最高达92.5%；愈伤组织进行继代培养的最佳培养基上是含有1.0mg/L 2, 4-D 和 0.1mg/L KT的MS培养基；最佳的悬浮培养基是含有1.0mg/L 2, 4-D 和0.1mg/L KT的MS培养基；最佳的悬浮系继代时间为16d；悬浮系中的成熟胚在基本培养基上可以发育成正常的植株。所建立的悬浮系可以用来进行小麦基因工程遗传改良和体细胞无性系突变体的筛选。

关键词: 小麦, 郑麦9023, 胚性悬浮细胞系, 植株再生

CLC Number:

S512.1

李冬兵，吕桂珍，牛洪斌，尹钧. 郑麦9023胚性悬浮细胞系的建立和植株再生[J]. 河南农业科学, 2018, 47(3): 34-37.

References

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郑麦9023胚性悬浮细胞系的建立和植株再生

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