doi:10.3969/j.issn.1004-3268.2007.09.013

Journal of Henan Agricultural Sciences ›› 2007, Vol. 36 ›› Issue (9): 45-47,51 .DOI: 10.3969/j.issn.1004-3268.2007.09.013

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Published:2007-09-15 Online:2007-09-15

RACE方法在甘蓝型油菜基因克隆中的应用

许本波;谢伶俐;严寒;何勇;田志宏

长江大学生命科学学院,湖北荆州434025

基金资助:
长江大学博士科研启动基金项目

Abstract

摘要： 将Invirtogen公司开发的一种RACE技术应用于甘蓝型油菜基因家族成员的克隆,克隆了查尔酮异构酶基因家族的全部6个成员和类黄酮3′-羟化酶基因家族的2个成员。应用结果表明：该方法具有低成本、高效率、能克隆全长基因的特点,适合甘蓝型油菜等多倍体物种基因家族成员的克隆。

关键词: 多基因家族, 克隆, RACE, 甘蓝型油菜

CLC Number:

S565.4

许本波;谢伶俐;严寒;何勇;田志宏. RACE方法在甘蓝型油菜基因克隆中的应用[J]. 河南农业科学, 2007, 36(9): 45-47,51 .

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RACE方法在甘蓝型油菜基因克隆中的应用

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