doi:10.3969/j.issn.1004-3268.2005.08.029

Journal of Henan Agricultural Sciences ›› 2005, Vol. 34 ›› Issue (8): 88-91.DOI: 10.3969/j.issn.1004-3268.2005.08.029

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Published:2005-07-15 Online:2005-07-15

IBDV VP3结构蛋白在大肠杆菌中的表达与鉴定

张改平,席俊,王选年,乔宏兴,张华,王丽,郭军庆

河南省动物免疫学重点实验室,河南省动物免疫学重点实验室,河南省动物免疫学重点实验室,河南省动物免疫学重点实验室,河南省动物免疫学重点实验室,河南省动物免疫学重点实验室,河南省动物免疫学重点实验室河南郑州450002,河南郑州450002,河南郑州450002,河南科技学院动物科学学院,河南新乡453003,河南郑州450002,河南郑州450002,河南郑州450002,河南郑州450002

基金资助:
中国科学院资助项目，河南省高校创新人才培养项目

Abstract

摘要： 应用RT-PCR技术从鸡IBDV总RNA中克隆出893bp的VP3基因,并将其进一步克隆于pET-28a载体T7启动子的下游,构建了pETVP3原核表达载体。经IPTG诱导,在其宿主菌BL21(DE3)中成功表达了35.5,33kDa的鸡IBDVVP3蛋白。经SDS-PAGE和Westernblotting分析,VP3表达产物以包涵体形式存在,并与鸡IBDV抗血清特异性反应。

关键词: 传染性法氏囊病病毒, VP3蛋白, 表达, 抗原性

CLC Number:

张改平,席俊,王选年,乔宏兴,张华,王丽,郭军庆. IBDV VP3结构蛋白在大肠杆菌中的表达与鉴定[J]. 河南农业科学, 2005, 34(8): 88-91.

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IBDV VP3结构蛋白在大肠杆菌中的表达与鉴定

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