doi:10.3969/j.issn.1004-3268.2010.08.034

Journal of Henan Agricultural Sciences ›› 2010, Vol. 39 ›› Issue (8): 126-129.DOI: 10.3969/j.issn.1004-3268.2010.08.034

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Published:2010-08-15 Online:2010-08-15

家蚕糖转运蛋白BmST5基因的克隆与转录活性检测

孔卫青;杨金宏;

安康学院陕西省蚕桑重点实验室;

基金资助:
陕西省自然科学基金项目(SJ08C108)

Abstract

摘要： 根据GenBank已登陆的糖转运蛋白序列与家蚕基因组框架图和EST序列,获得家蚕同源糖转运蛋白基因BmST5。基因编码区长1 527 bp,编码508个氨基酸,预测蛋白分子质量为55.67 kD。序列分析结果显示,该基因有典型的Sugar_tr结构域和12个疏水的跨膜结构域,与体虱等同源蛋白的一致性在50%以上。RT-PCR结果表明,该基因仅在家蚕的马氏管、生殖腺和头中有表达,在中肠、丝腺、血液、体壁和脂肪体中不表达。

关键词: 家蚕, 糖转运蛋白, BmST5基因, 克隆

CLC Number:

孔卫青;杨金宏;. 家蚕糖转运蛋白BmST5基因的克隆与转录活性检测[J]. 河南农业科学, 2010, 39(8): 126-129.

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家蚕糖转运蛋白BmST5基因的克隆与转录活性检测

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