Effects of Saline‑Alkali Stress on Vegetative and Reproductive Growth of Japonica Rice Germplasm Resource

doi:10.15933/j.cnki.1004-3268.2025.08.003

Abstract

Abstract: Using 164 japonica rice germplasm resources as test materials，the effects of saline‑alkali stress［normal paddy soil（control，CK），alkalized saline soil（T1）and alkali soil（T2）］on plant height（PH），tillers number per plant（NT），basal stem diameter（SD），days from sowing to heading（S‑FHD），effective panicles number per plant（PNPP），total grains number per plant（SPP），1 000‑grain weight（TGW），seed setting rate（SSR） and yield per plant（YPP） were studied under field conditions.Comprehensive evaluation was conducted by combining correlation analysis，principal component analysis and membership function to screen saline‑alkali tolerant germplasms during the whole growth period.The key indicators affecting the vegetative and reproductive growth of rice under different saline‑alkali stresses were screened through linear regression analysis，so as to provide theoretical basis for breeding
saline‑alkali tolerant and high‑yielding rice varieties.The results showed that under the T1 and T2 treatments，PH，SPP and YPP of the japonica rice germplasm were significantly lower than those of CK.The differences of the above 9 traits between T1 and T2 treatments were all significant.The correlation analysis results showed that PH，SPP and S‑FHD were significantly or extremely significantly positively correlated with each other，and PH was significantly or extremely significantly positively correlated with TGW and YPP under all treatments. Under T1 and T2 treatments，PNPP was significantly or extremely significantly negatively correlated with PH and S‑FHD，while TGW was significantly or extremely significantly positively correlated with SSR and YPP.However，the correlations of the above traits did not reach significant levels under CK.Principal component analysis results showed that 4 principal components were extracted under CK，T1 and T2 treatments，with cumulative contribution rates of 79.550%，82.530% and 79.076%，respectively.Combined the key factor load values of each principal component with the R² values of the linear regression equations between the 9 traits and the comprehensive saline‑alkali tolerance D value，PH，SPP and S‑FHD were determined as the key indicators affecting the vegetative and reproductive growth of japonica rice germplasms under saline‑alkali stress.According to the D value，two saline‑alkali tolerant germplasms，Liujing 2 and Bijing 45，were screened.

Key words: Japonica rice, Saline?alkali stress, Vegetative growth, Reproductive growth, Linear regression analysis

摘要： 以164份粳稻种质资源为试验材料，在大田环境下种植，研究盐碱胁迫［正常稻田土（对照，CK）、碱化盐土（T1）和碱土（T2）］对水稻株高（PH）、单株分蘖数（NT）、茎基粗（SD）、播始历期（S-FHD）、单株有效穗数（PNPP）、单株总粒数（SPP）、千粒质量（TGW）、结实率（SSR）、单株产量（YPP）的影响，结合相关性分析、主成分分析、隶属函数等进行综合评价，筛选全生育期耐盐碱种质，并通过线性回归分析筛选出不同盐碱胁迫下影响水稻营养生长和生殖生长的关键指标，为培育耐盐碱高产水稻品种提供理论依据。结果表明，在T1和T2处理下，粳稻种质的PH、SPP、YPP均显著低于CK；T1和T2处理间上述9个性状的差异均达到显著水平。相关性分析结果表明，PH、SPP、S-FHD间及PH与TGW、YPP等在所有处理下均呈显著或极显著正相关；在T1和T2处理下，PNPP与PH、S-FHD均呈显著或极显著负相关，TGW与SSR、YPP均呈显著或极显著正相关，但在CK下以上性状的相关性均未达到显著水平。主成分分析结果表明，在CK、T1 和T2 处理下均提取到4 个主成分，累计贡献率分别为79.550%、82.530% 和79.076%。结合各主成分关键因子载荷值和9个性状与综合耐盐碱能力D值的线性回归方程的R²值确定PH、SPP和S-FHD是影响盐碱胁迫下粳稻种质营养生长、生殖生长的关键指标。依据D值筛选出六粳2号和毕粳45号2份耐盐碱种质。

关键词: 粳稻, 盐碱胁迫, 营养生长, 生殖生长, 线性回归分析

CLC Number:

S511

DUAN Kairong, RAN Jie, SONG Jiawei, QIAO Chengbin, KONG Weiru, DONG Yue, LIU Songrui, ZHANG Bi, XU Hao, MA Donghua, LI Peifu, LUO Chengke, TIAN Lei. Effects of Saline‑Alkali Stress on Vegetative and Reproductive Growth of Japonica Rice Germplasm Resource[J]. Journal of Henan Agricultural Sciences, 2025, 54(8): 26-37.

段凯蓉, 冉杰, 宋佳伟, 乔承彬, 孔维儒, 董悦, 刘松瑞, 张碧, 许昊, 马东花, 李培富, 罗成科, 田蕾. 盐碱胁迫对粳稻种质资源营养与生殖生长的影响[J]. 河南农业科学, 2025, 54(8): 26-37.

Figures/Tables 6

References

［1］EPSTEIN E，NORLYN J D，RUSH D W，et al. Saline culture of crops：A genetic approach［J］. Science，1980，210（4468）：399‑404.
［2］GUO S Q，CHEN Y X，JU Y L，et al. Fine‑tuning gibberellin improves rice alkali‑thermal tolerance and yield［J］. Nature，2025，639（8053）：162‑171.
［3］ADAM D. How far will global population rise？Researchers can’t agree［J］. Nature，2021，597（7877）：462‑465.
［4］XU X，GUO L，WANG S B，et al. Effective strategies for reclamation of saline‑alkali soil and response mechanisms of the soil‑plant system［J］. Science of the Total Environment，2023，905：167179.

［5］马国辉，郑殿峰，母德伟，等. 耐盐碱水稻研究进展与展望［J］. 杂交水稻，2024，39（1）：1‑10.

MA G H，ZHENG D F，MU D W，et al. Research progress and prospect of saline‑alkali tolerant rice［J］.Hybrid Rice，2024，39（1）：1‑10.
［6］LIU B S，HUANG L H，JIANG X T，et al. Quantitative evaluation and mechanism analysis of soil chemical factors affecting rice yield in saline‑sodic paddy fields［J］.Science of the Total Environment，2024，929：172584.

［7］GONG B，LI X，VANDENLANGENBERG K M，et al.Overexpression of S‑adenosyl‑l‑methionine synthetase increased tomato tolerance to alkali stress through polyamine metabolism［J］.Plant Biotechnology Journal，2014，12（6）：694‑708.

［8］YU J，ZHU C S，XUAN W，et al. Genome‑wide association studies identify OsWRKY53 as a key regulator of salt tolerance in rice［J］. Nature Communications，2023，14（1）：3550.
［9］周根友，汪娟，赵祥强. 大田评价水稻耐盐碱性的农艺性状指标研究［J］.华北农学报，2017，32（S1）：102‑107.
ZHOU G Y，WANG J，ZHAO X Q. Field indexes of agronomic traits for salinity tolerance evaluation in rice［J］.Acta Agriculturae Boreali‑Sinica，2017，32（S1）：102‑107.

［10］ZHANG Q F. Strategies for developing green super rice［J］.Proceedings of the National Academy of Sciences of the United States of America，2007，104（42）：16402‑16409.

［11］PENG S B，KHUSH G S，VIRK P，et al. Progress in ideotype breeding to increase rice yield potential［J］.Field Crops Research，2008，108（1）：32‑38.

［12］王瑞云，郭琦，王计平，等. 水稻营养生长阶段系统的划分［J］.中国农学通报，2011，27（5）：89‑93.

WANG R Y，GUO Q，WANG J P，et al. Highlights in theVegetable stage system of rice（Oryza sativa L.）［J］. Chinese Agricultural Science Bulletin，2011，27（5）：89‑93.

［13］YADAV S P，BHARADWAJ R，NAYAK H，et al.Impact of salt stress on growth，productivity and physicochemical properties of plants：A review［J］.International Journal of Chemical Studies，2019，7（2）：1793‑1798.

［14］ZHANG Z H，GAO S P，CHU C C. Improvement of nutrient use efficiency in rice：Current toolbox and future perspectives［J］.Theoretical and Applied Genetics，2020，133（5）：1365‑1384.

［15］HUSSAIN S，ZHANG J H，ZHONG C，et al. Effects of salt stress on rice growth，development characteristics，and the regulating ways：A review［J］.Journal of Integrative Agriculture，2017，16（11）：2357‑2374.

［16］LUTTS S，KINET J M，BOUHARMONT J. Changes in plant response to NaCl during development of rice（Oryza sativa L.） varieties differing in salinity resistance［J］. Journal of Experimental Botany，1995，46 （12）：1843‑1852.

［17］ZENG L H，SHANNON M C. Salinity effects on seedling growth and yield components of rice［J］.Crop Science，2000，40（4）：996‑1003.

［18］梁正伟，杨福，王志春，等. 盐碱胁迫对水稻主要生育性状的影响［J］.生态环境，2004，13（1）：43‑46.

LIANG Z W，YANG F，WANG Z C，et al. Effect of the main growth characteristics of rice under saline‑alkali stress［J］. Ecology and Environmental Sciences，2004，13（1）：43‑46.

［19］王志春，杨福，齐春艳，等. 盐碱胁迫下水稻渗透调节的生理响应［J］.干旱地区农业研究，2010，28（6）：153‑157.

WANG Z C，YANG F，QI C Y，et al. Osmotic regulation response of rice to soil salinity and alkalinity stresses［J］.Agricultural Research in the Arid Areas，2010，28（6）：153‑157.

［20］ALI S，GAUTAM R K，MAHAJAN R，et al. Stress indices and selectable traits in SALTOL QTL introgressed rice genotypes for reproductive stage tolerance to sodicity and salinity stresses［J］. Field Crops Research，2013，154：65‑73.

［21］BALASUBRAMANIAM T，SHEN G X，ESMAEILI N，et al. Plants’response mechanisms to salinity stress［J］.Plants，2023，12（12）：2253.

［22］孔维儒，宋佳伟，段凯蓉，等. 盐碱胁迫对粳稻种质资源穗部形态建成的影响［J］. 植物遗传资源学报，2025，26（1）：90‑107.
KONG W R，SONG J W，DUAN K R，et al. Effects of saline‑alkaline stress on the morphogenesis of panicle
of japonica rice germplasm［J］.Journal of Plant Genetic Resources，2025，26（1）：90‑107.

［23］LIU C T，MAO B G，YUAN D Y，et al. Salt tolerance in rice： Physiological responses and molecular mechanisms［J］. The Crop Journal，2022，10（1）：13‑25.

［24］WANG B，SMITH S M，LI J Y. Genetic regulation of shoot architecture［J］. Annual Review of Plant Biology，2018，69：437‑468.

［25］HE Q，WU H，ZENG L J，et al. OsKANADI1 and OsYABBY5 regulate rice plant height by targeting GIBERELLIN 2‑OXIDASE6［J］. The Plant Cell，2024，37（1）：koae276.

［26］ LÜ B S，LI X W，MA H Y，et al. Differences in growth and physiology of rice in response to different saline‑alkaline stress factors［J］. Agronomy Journal，2013，105（6）：1889.

［27］于艳敏，吴立成，武洪涛，等. 盐碱胁迫对水稻生长特性及产量的影响［J］.黑龙江农业科学，2024（3）：1‑5.
YU Y M，WU L C，WU H T，et al. Effects of saline‑alkali stress on growth characteristics and yield of rice［J］. Heilongjiang Agricultural Sciences，2024（3）：1‑5.

［28］DU A P，TIAN W，WEI M H，et al. The DTH8‑Hd1 module mediates day‑length‑dependent regulation of rice flowering［J］. Molecular Plant，2017，10（7）：948‑961.

［29］ SONG Y H，SHIM J S，KINMONTH‑SCHULTZ H A，et al. Photoperiodic flowering：Time measurement mechanisms in leaves［J］. Annual Review of Plant Biology，2015，66：441‑464.

［30］WANG X L，HE Y Q，WEI H，et al. A clock regulatory module is required for salt tolerance and control of heading date in rice［J］. Plant，Cell & Environment，2021，44（10）：3283‑3301.

［31］AREF F. Physiological characterization of rice under salinity stress during vegetative and reproductive stages［J］. Indian Journal of Science and Technology，2012，5 （4）：1‑9.

［32］KHAN M A，ABDULLAH Z. Salinity‑sodicity induced changes in reproductive physiology of rice（Oryza sativa）under dense soil conditions［J］. Environmental and Experimental Botany，2003，49（2）：145‑157.

［33］GIMHANI D R，GREGORIO G B，KOTTEARACHCHI N S，et al. SNP‑based discovery of salinity‑tolerant QTLs in a bi‑parental population of rice（Oryza sativa）［J］. Molecular Genetics and Genomics，2016，291（6）：2081‑2099.

［34］WEI H，WANG X M，ZHANG Z P，et al. Uncovering key salt‑tolerant regulators through a combined eQTL and GWAS analysis using the super pan‑genome in rice［J］. National Science Review，2024，11（4）：nwae043.

［35］齐春艳，梁正伟，杨福. 苏打盐碱胁迫下水稻抽穗期的变化规律及其影响因素的研究［J］.农业系统科学与综合研究，2009，25（2）：198‑203.
QI C Y，LIANG Z W，YANG F. Changes of rice heading date and their influencing factors under saline‑alkali stress［J］. System Sciences and Comprehensive Studies in Agriculture，2009，25（2）：198‑203.

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