The Relationship between Color Change and Energy Metabolism of Tobacco Leaves after Harvest in Different Leaf Positions

doi:10.15933/j.cnki.1004-3268.2022.04.019

Abstract

Abstract: This research aimed to study the relationship between color change and energy metabolism of tobacco leaves after harvest in different leaf positions. By choosing tobacco leaves with different leaf positions as the research object，the yellowing and browning process of postharvest tobacco leaves was analyzed by dark test.The yellowing degree，browning degree，malondialdehyde content，sugar（total sugar，reducing sugar，starch）content，energy metabolism‑related substances（ATP，ADP，AMP）content，energy charge and cytochrome oxidase（CCO），succinate dehydrogenase（SDH） activities were determined during experiment.The research results showed that：（1）As the leaf position was elevated，the yellowing and browning of tobacco leaves were slower with the lower yellowing index and browning index，and the MDA content and the conductivity of the extract increased more slower.（2） In comparison，the starch content of the middle and upper leaves was higher and the degradation peak was later，and the total sugar and reducing sugar content maintained at high level in the middle and late stages.（3）Compared with the lower leaves，the ATP content and energy charge level of the middle and upper leaves were higher and the peaks appeared later，and CCO and SDH activities were relatively higher.（4）The degree of yellowing of tobacco leaves showed a significant positive correlation with total sugar，reducing sugar content and energy charge（P<0.05），respectively；the degree of browning showed a significant negative correlation with reducing sugar and ADP content（P<0.05），respectively.In summary，the yellowing and browning of the middle and upper leaves develop more slowly than the lower leaves.The difference in color changes of tobacco leaves in different leaf positions may be related to their energy metabolism activities.

Key words: Flue?cured tobacco, Leaf position, Curing characteristics, Postharvest physiology, Sugar content, Energy metabolism, Color change

摘要： 为明确不同部位采后烟叶颜色变化差异与能量代谢的关系，以云烟87不同部位烟叶为研究对象，采用暗箱试验的方式，分析采后烟叶的变黄变褐过程，并对试验过程中烟叶的变黄程度、变褐程度、丙二醛（MDA）含量、糖（总糖、还原糖、淀粉）含量、能量代谢相关物质（ATP、ADP、AMP）含量、能荷和细胞色素氧化酶（CCO）、琥珀酸脱氢酶（SDH）等呼吸代谢酶活性进行测定。结果表明：（1）随着部位的提升，烟叶变黄变褐更慢，变黄指数和变褐指数更低，MDA含量和浸出液电导率增加速度也更缓慢。（2）相较而言，中、上部叶的淀粉含量更高，降解高峰更靠后，其总糖和还原糖含量在中后期维持较高水平。（3）与下部叶相比，中、上部叶的ATP含量及能荷水平更高，峰值出现更晚，其CCO和SDH活性相对较高。（4）烟叶的变黄程度与总糖、还原糖含量及能荷呈显著的正相关关系（P<0.05）；变褐程度与还原糖、ADP含量呈显著的负相关关系（P<0.05）。综上，中、上部叶变黄变褐程度较下部叶发展更慢，不同部位烟叶颜色变化差异可能与其能量代谢活动有关。

关键词: 烟叶, 部位, 烘烤特性, 采后生理, 糖含量, 能量代谢, 颜色变化

CLC Number:

S572

NIU Hao, LI Jinghao, LI Pengcheng, SUN Guangwei, BAI Tao, CHEN Zhenguo, SONG Zhaopeng, WANG Tao. The Relationship between Color Change and Energy Metabolism of Tobacco Leaves after Harvest in Different Leaf Positions[J]. Journal of Henan Agricultural Sciences, 2022, 51(4): 171-180.

牛浩, 李静浩, 李鹏程, 孙光伟, 白涛, 陈振国, 宋朝鹏, 王涛. 不同部位采后烟叶颜色变化差异与能量代谢的关系[J]. 河南农业科学, 2022, 51(4): 171-180.

Figures/Tables 7

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