Abstract: SQUAMOSA PROMOTER BINDING PROTEIN-LIKE ( SPL) protein is plant-specific transcription factor,play vital roles in the regulatory responses of plant growth,development and stress.In this study,15 SPL genes were identified from Morus notabilis. MnSPLs transcription factors were clustered into eight groups.Gene structure and motif analysis indicated that most MnSPLs had relatively conserved exonintron arrangements and amino acids motif composition.Several paralogs and orthologs were predicted within the MnSPLs and along with Arabidopsis thaliana and Oryza sativa SPLs.Seven MnSPLs were predicted to be targets of miR156/157.MnSPLs were expressed differentially in various tissues of M.notabilis,six genes of them were highly expressed in five tissue,some genes were mainly highly expressed in winter buds and male flowers.MnSPL genes tissue-specific expression,and played important role in the growth and development of mulberry.

Key words: Mulberry, Morus notabilis, SPL gene, Transcription factor, miR156/157, Gene expression

摘要： SQUAMOSA启动子结合类蛋白质（SQUAMOSA promoter binding protein-like，SPL）是一类植物生长发育和应对胁迫相关的重要转录因子。利用生物信息学方法，从川桑（Morus notabilis）基因组中鉴定到15个SPL基因，进化分析将其归为8个聚类组，其具有保守的基因结构和氨基酸基序，在拟南芥和水稻中存在多个直系同源基因，预测到7个MnSPLs基因的表达受miR156/157调控，MnSPLs在桑树多个组织中检测到表达，其中6个基因在5类组织中均有较高表达量，部分基因主要在冬芽和雄花中有较高表达量，MnSPL基因存在组织表达特异性，在桑树生长发育过程中发挥重要作用。

关键词: 桑树；川桑, SPL基因；转录因子；miR156/157；基因表达