doi:10.3969/j.issn.1004-3268.2010.07.028

Journal of Henan Agricultural Sciences ›› 2010, Vol. 39 ›› Issue (7): 97-103.DOI: 10.3969/j.issn.1004-3268.2010.07.028

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Published:2010-07-15 Online:2010-07-15

白羽番鸭脂联素基因内含子多态与肉质的关联分析

张依裕^1,2;徐琪¹;段修军³;张海波¹;赵文明¹;孙国波³;陈国宏¹

¹扬州大学动物科学与技术学院,江苏扬州225009;²贵州大学动物科学学院,贵州贵阳550025;³江苏畜牧兽医职业技术学院,江苏泰州225300

基金资助:
基金项目：国家科技支撑计划重大专项（2006BDA01A09）；国家科技支撑计划重点项目（2006BDA14806）；现代农业产业技术体系建设专项基金（nvcytx-45-04）

Abstract

摘要： 采用PCR-SSCP法对白羽番鸭脂联素基因内含子进行SNP检测,并分析SNP对肉质的遗传效应。结果表明,检测到3种基因型（CC、CT和TT）和2个等位基因（C和T）,CT和C分别为优势基因型和优势等位基因。序列比对发现,在内含子内存在1个SNP位点T290C,该位点在白羽番鸭群体中表现为中度多态,处于Hardy-Weinberg平衡状态（P〉0.05）;最小二乘法分析表明,CC基因型个体的IMF显著高于TT基因型个体（P〈0.05）,失水率极显著低于TT基因型个体（P〈0.01）;CT基因型个体的失水率显著低于TT基因型个体（P〈0.05）。结果揭示,T290C位点可能对脂肪沉积有显著影响,CC基因型是白羽番鸭肉质较为优良的基因型。

关键词: 白羽番鸭, 脂联素基因, PCR-SSCP, 关联分析

CLC Number:

S834

张依裕;徐琪;段修军;张海波;赵文明;孙国波;陈国宏. 白羽番鸭脂联素基因内含子多态与肉质的关联分析[J]. 河南农业科学, 2010, 39(7): 97-103.

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白羽番鸭脂联素基因内含子多态与肉质的关联分析

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