doi:10.3969/j.issn.1004-3268.2008.02.025

Journal of Henan Agricultural Sciences ›› 2008, Vol. 37 ›› Issue (2): 88-90.DOI: 10.3969/j.issn.1004-3268.2008.02.025

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Published:2015-02-15 Online:2015-02-15

新郑灰枣基因组DNA提取方法比较

李振山;王珂;李海涛;张志阳;冯建灿

河南农业大学林学园艺学院,河南郑州450002

Abstract

摘要： 以新郑灰枣苗叶片为材料，采用CTAB方法提取叶片DNA，并对该方法进行改良。比较4种DNA提取方式，对提取结果进行紫外分光光度、电泳、酶切等方法的鉴定，结果表明，提取的灰枣叶片DNA得率约为0．370～0．530μtg／mg，A260／A280约为1．90～2．00，A260／A230在2．00以上，分子量在23kb以上，能够被EcoRⅠ，HindⅢ酶切。其中，第2种处理方法提取效果较好。

关键词: 灰枣, DNA提取, 限制性酶切

CLC Number:

S665.1

李振山;王珂;李海涛;张志阳;冯建灿. 新郑灰枣基因组DNA提取方法比较[J]. 河南农业科学, 2008, 37(2): 88-90.

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新郑灰枣基因组DNA提取方法比较

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