王守刚;王永岐;沈阿林;吕爱英

摘要： 采用CRY-3型细胞融合仪,在改变电场强度,其他电融合参数不变的情况下处理供试固氮菌(N5-1)和磷钾菌(N17)亲本.结果表明,在电场强度为3.09kV/cm时,细胞致死率最接近50%,融合率最高.该条件下获得融合子62株,融合率为7.8×10-4%.

关键词: 细胞电融合技术, 选育, 固氮菌, 磷钾菌, 致死率, 抗药性, 融合子

CLC Number:

王守刚;王永岐;沈阿林;吕爱英. 利用细胞电融合技术选育固氮菌、磷钾菌的方法探讨[J]. 河南农业科学, 2004, 33(4): 43-45.

[1]	HE Jianqing, ZHANG Gejie. Effects of Plant Growth Promoting Rhizobacteria（PGPR）Fertilizer instead of Part of Chemical Fertilizer on Growth，Yield and Quality of Black Highland Barley [J]. Journal of Henan Agricultural Sciences, 2022, 51(7): 93-101.
[2]	CHEN Xiaojia, LI Li, MA Xinxin, SHI Jiping, ZHAO Zhijun. Screening and Application of Thermophilic Bacteria for Degrading Soybean Dregs [J]. Journal of Henan Agricultural Sciences, 2021, 50(1): 172-179.
[3]	. [J]. Journal of Henan Agricultural Sciences, 2010, 39(12): 48-51.
[4]	. [J]. Journal of Henan Agricultural Sciences, 2004, 33(4): 34-36.
[5]	. [J]. Journal of Henan Agricultural Sciences, 2004, 33(4): 37-39.
[6]	. [J]. Journal of Henan Agricultural Sciences, 2004, 33(4): 40-42.
[7]	. [J]. Journal of Henan Agricultural Sciences, 2004, 33(4): 46-49.
[8]	. [J]. Journal of Henan Agricultural Sciences, 2004, 33(4): 49-51.
[9]	. [J]. Journal of Henan Agricultural Sciences, 2004, 33(4): 52-53.
[10]	. [J]. Journal of Henan Agricultural Sciences, 2000, 29(8): 20-21.

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