doi:10.3969/j.issn.1004-3268.2007.03.031

Journal of Henan Agricultural Sciences ›› 2007, Vol. 36 ›› Issue (3): 109-111.DOI: 10.3969/j.issn.1004-3268.2007.03.031

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Published:2007-03-15 Online:2007-03-15

法氏囊B细胞λ轻链基因编码蛋白在大肠杆菌中的表达

宋海涛^1,2;张红^1,2,3;王丽²;段艳华^1,2;乔松林^1,2;李学伍²;赵光辉^1,2;张改平²

¹河南农业大学,河南郑州450002;²河南省动物免疫学重点实验室,河南郑州450002;³河南教育学院,河南郑州450003

基金资助:
国家自然科学基金重点项目（30203270）

Abstract

摘要： 为获得鸡λ轻链基因的表达蛋白，采用聚合酶链反应从鸡法氏囊B细胞cDNA文库中扩增出分泌表达蛋白的编码基因；用限制性内切酶消化后，插入克隆载体pUC19中。经PCR鉴定与序列测定证实后，以亚克隆法构建于原核表达载体PET43a的相应酶切位点。经过基因序列测定、BamHⅠ和Hind Ⅲ双酶切鉴定证实克隆载体正确插入载体PET43a。转化宿主菌BL21（DE3），经IPTG诱导、SDS—PAGE分析表明目的蛋白得到表达；且能与His—Taq单抗相结合。

关键词: 传染性法氏囊, 基因, 蛋白, 表达

CLC Number:

S858.31

宋海涛;张红[1,2,3];王丽;段艳华;乔松林;李学伍;赵光辉;张改平. 法氏囊B细胞λ轻链基因编码蛋白在大肠杆菌中的表达[J]. 河南农业科学, 2007, 36(3): 109-111.

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法氏囊B细胞λ轻链基因编码蛋白在大肠杆菌中的表达

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