Abstract: In order to solve the technical problems of low extraction efficiency of traditional Chinese herb，single factor experiment combined with response surface methodology was carried out to improve thepreparation process of livestock and poultry Qingfei granules，including fermentation extraction，extract extraction，alcohol precipitation，granule formulation，granulation，drying and straightening granulation，etc.，and the content of active ingredients of the modified livestock and poultry Qingfei granules was determined.The results showed that the optimal preparation conditions of livestock and poultry Qingfei granules were as follows：inoculation ratio was 3%，culture temperature was 37℃ and culture time was 72 h；the fermented traditional Chinese medicine was extracted twice with 8 times of water，1.5 h each time；the extractum was soaked in 70% ethanol under the condition of relative density of 1.35 for 24 h；the extractum was mixed with auxiliary materials at a ratio of 1∶7.6，and the auxiliary materials were lactose and dextrin with the ratio of 1∶2；the wet mixing time was 30 min with 80% ethanol，and then they were passed through a 1.40 mm aperture sieve，and mixed for 30 min；the wet particles were dried at 70℃ for 4 h and coarse granules could be prepared with a 1.40 mm aperture sieve of a rocking granulator. Compared with traditional livestock and poultry Qingfei granules，the contents of epigoitrin and quercetin 3⁃O⁃β⁃D⁃glucose⁃7⁃O⁃β⁃D⁃gentiobioside were increased by 22.67% and 25.39% respectively，and the contents of liquiritin and ammonium glycyrrhizinate were doubled in modified livestock and poultry Qingfei granules.

Key words: Livestock and poultry Qingfei granule, Response surface method, Herb fermentation, Preparation process

摘要： 为了解决传统中药提取效率低的技术问题，采用单因素试验结合响应面法对畜禽清肺颗粒制备工艺进行改良，包括发酵提取、浸膏提取、醇沉、颗粒处方、制粒、烘干和整粒等，并测定改良畜禽清肺颗粒的药物有效成分含量。结果表明，畜禽清肺颗粒最佳制备工艺为发酵中药加8 倍水提取2 次，1.5 h/次；所得浸膏用70%乙醇于相对密度1.35条件下静置24 h；将浸膏、辅料以1∶7.6配比，辅料为乳糖与糊精1∶2配比，并以80%乙醇作为湿润剂湿混30 min，过1.40 mm孔径筛，总混30 min，制备颗粒；湿颗粒于70 ℃烘干4 h，用1.40 mm孔径漩振筛整粒，粗颗粒用摇摆颗粒机1.40 mm孔径筛继续整粒即可。与传统畜禽清肺颗粒相比，改良畜禽清肺颗粒中（R，S）-告依春和槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷含量分别提高22.67%和25.39%，甘草苷和甘草酸铵含量均提高1倍以上。

关键词: 畜禽清肺颗粒, 响应面法, 中药发酵, 制备工艺