Abstract: In order to clarify the types of the pathogens of fruit rot disease of main kiwifruit varieties in Guizhou Province,and provide theoretical basis for the prevention and control of field fruit diseases,the diseased fruits of Hongyang and Guichang kiwifruit collected in Shuicheng County and Xiuwen County of Guizhou Province from 2018 to 2019 were used as experimental materials.The pathogens were identified through pathogen isolation,morphological observation,molecular detection and pathogenicity detection.The phylogenetic analysis of pathogen species based on their sequences were carried out.The results showed that four kinds of pathogens including Phomopsis cauloides, Fusarium, Alternaria alternata,Plectosphaerella cucumerina were isolated, and their detection rates were 10%, 60%, 20%, 10%respectively. The fruit disease of kiwifruit caused by the infection of Plectosphaerella cucumerina was reported for the first time in China.

Key words: Kiwifruit, Canker disease, Pathogen identification, Sequence analysis, Pathogenicity test, Phomopsis cauloides, Fusarium, Plectosphaerella cucumerina

摘要： 为明确贵州省猕猴桃主栽品种果实腐烂病病原菌的种类，并为田间果实病害防治提供理论依据，以2018—2019年在贵州省水城县和修文县两大主产区采集的红阳和贵长猕猴桃病果为试验材料，通过病原菌的分离、形态学观察、分子检测和致病性检测，研究病果中病原真菌的种类，并对其序列进行系统发育分析。结果表明，分离到4类病原菌：拟茎点霉菌（Phomopsis cauloides）、镰刀菌（Fusarium）、交链格孢菌（Alternaria alternata）、黄瓜织球壳菌（Plectosphaerella cucumerina)，其检出率分别为10%、60%、20%、10%。其中，黄瓜织球壳菌侵染引起猕猴桃果实病害在国内尚属首次报道。

关键词: 猕猴桃； 腐烂病； 病原菌鉴定； 序列分析； 致病性检测, 拟茎点霉菌； 镰刀菌； 黄瓜织球壳菌