Identification of a Cellulase Producing Fungus from Tibet Hot
 Spring and Optimization of Its Enzyme Production Conditions

doi:10.15933/j.cnki.1004-3268.2019.10.012

Journal of Henan Agricultural Sciences ›› 2019, Vol. 48 ›› Issue (10): 77-83.DOI: 10.15933/j.cnki.1004-3268.2019.10.012

• Agricultural Resources and Environment • Previous Articles Next Articles

Identification of a Cellulase Producing Fungus from Tibet Hot Spring and Optimization of Its Enzyme Production Conditions

LU Yuxin¹,ZHAO Hangke¹,TANG Xiaofei¹,LIU Kaihui^1,2,DENG Baiwan^1,3,DING Xiaowei¹

( 1.School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China; 2.School of Food and Biological Engineering,Shaanxi University of Science ＆ Technology,Xi’an 710021,China; 3.Shaanxi Provincial Engineering Ｒesearch Center of Edible and Medicinal Fungi,Hanzhong 723000,China)

Received:2019-01-18 Published:2019-10-15 Online:2019-10-15

西藏热泉1株产纤维素酶真菌的鉴定及产酶条件优化

卢雨欣¹，赵航轲¹，唐小飞¹，刘开辉^1,2，邓百万^1,3，丁小维^1,2

（1.陕西理工大学生物科学与工程学院，陕西汉中 723000；2.陕西科技大学食品与生物工程学院，陕西西安 710021；3.陕西省食药用菌工程技术研究中心，陕西汉中 723000）

通讯作者: 刘开辉（1979-），男，陕西旬阳人，副教授，博士，主要从事特殊环境微生物资源开发与利用研究。E-mail:kaihhui168@hotmail.com
作者简介:卢雨欣（1995-），女，陕西咸阳人，在读硕士研究生，研究方向：微生物资源保育及开发利用。E-mail:490631724@qq.com
基金资助:
陕西省科技厅重点研发计划项目（2018NY-156）

Abstract

Abstract: In order to deepen the development and utilization of thermophilic cellulase resources,a cellulase producing fungus THN8 from Nimu hot spring in Tibet was isolated and identified，and the cellulase producing activity of this strain was screened by Congo red culture medium.According to the morphology and ITS sequence analysis,the strain THN8 was identified as Melanocarpus albomyces.On the basis of single factor experiment,orthogonal experiment was used to optimize the cellulase producing conditions of the strain THN8． Finally，the optimal medium formula for the cellulase producing strain THN8 was as follows: lactose 92.0 g/L,(NH₄)₂SO₄ 6.0 g/L，K₂HPO₄3.0 g/L，MgSO₄·7H₂O 0.55 g/L.The optimal fermentation conditions were as follows: the fermentation temperature was 58℃，the initial pH value was 5.5,and the fermentation time was 4 days. Under these conditions,the cellulase activity of the strain THN8 reached 28.1 U/mL.The thermophilic fungus isolated in this experiment plays an important role in the degradation and utilization of cellulose agricultural waste．

Key words: Tibet hot spring, Thermophilic fungi, Identification, Cellulase, Enzyme production condition

摘要： 为深化嗜热纤维素酶资源的开发利用，从西藏尼木热泉分离得到1株产纤维素酶嗜热真菌THN8，根据该菌株在刚果红培养基上产生的透明圈大小，初步判断其产纤维素酶能力。通过形态学和ITS序列分析,鉴定其为Melanocarpus albomyces。在单因素试验基础上采用正交试验确定菌株THN8产纤维素酶的最佳培养基配方及发酵条件，最终获得该菌株产纤维素酶的最佳培养基配方为乳糖92.0 g/L、(NH₄)₂SO₄6.0 g/L、K₂HPO4 3.0 g/L、MgSO₄·7H₂O 0.55 g/L，最佳发酵条件为发酵温度58℃、起始pH值5.5、发酵时间4 d，在此条件下菌株THN8的纤维素酶活力达到28.1 U/mL。试验分离出的产纤维素酶嗜热真菌在降解利用纤维素类农业废弃物方面具有重要作用。

关键词: 西藏热泉, 嗜热真菌, 鉴定, 纤维素酶, 产酶条件

CLC Number:

S154

LU Yuxin, ZHAO Hangke, TANG Xiaofei, LIU Kaihui, DENG Baiwan, DING Xiaowei. Identification of a Cellulase Producing Fungus from Tibet Hot Spring and Optimization of Its Enzyme Production Conditions[J]. Journal of Henan Agricultural Sciences, 2019, 48(10): 77-83.

卢雨欣, 赵航轲, 唐小飞, 刘开辉, 邓百万, 丁小维. 西藏热泉1株产纤维素酶真菌的鉴定及产酶条件优化[J]. 河南农业科学, 2019, 48(10): 77-83.

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Identification of a Cellulase Producing Fungus from Tibet Hot Spring and Optimization of Its Enzyme Production Conditions

西藏热泉1株产纤维素酶真菌的鉴定及产酶条件优化

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