SHORT VEGETATIVE PHASE(SVP)基因作为重要的开花抑制因子，受多种环境因子影响，参与多条花期调控途径。为了明确菊花CmSVP基因在花期调控中的功能，以日中性菊花金不凋为材料，通过PCR克隆CmSVP基因，分析CmSVP基因结构；采用qRT-PCR方法分析CmSVP在菊花不同时期的不同组织、不同光周期及不同温度条件下的表达模式。结果表明，CmSVP基因在gDNA上全长为4 997 bp，由8个外显子和7个内含子构成。CmSVP在营养时期的表达水平高于生殖生长时期，其中在生长点茎尖处表达丰度最高，花序的表达量最低，推测CmSVP可能抑制成花。CmSVP基因表现出对光周期和温度的敏感性，特别是长日照(LD)及低温的双重作用能够显著抑制其表达。综上，菊花CmSVP基因与其他物种SVP同源基因结构类似，主要在营养时期表达，对温度和光周期敏感，可能通过温敏途径及光周期途径抑制菊花成花。

As an important flowering inhibitor,SHORT VEGETATIVE PHASE(SVP) gene is affected by multiple environmental factors, and participates in multiple flowering regulation pathways.In order to clarify the function of CmSVP gene in the regulation of Chrysanthemum flowering,CmSVP gene of the dayneutral Chrysanthemum morifolium Jinbudiao was cloned by PCR,and the gene structure was analyzed.The expression patterns of CmSVP in different tissues in different periods,under different photoperiods and at different temperatures were detected by qRT-PCR.The results showed that the total length of CmSVP gDNA was 4 997 bp,which consisted of 8 exons and 7 introns.The expression level of CmSVP in the vegetative period was higher than that in the reproductive growth period.The expression abundance was highest at the shoot tip,and that of the inflorescence was the lowest.So it was speculated that CmSVP might inhibit flower formation.The CmSVP gene showed sensitivity to photoperiod and temperature,especially the dual effects of long day and low temperature could significantly inhibit its expression.These results indicated that the CmSVP was similar in structure to the SVP homologous genes of other species,and was mainly expressed in the vegetative period.It was speculated to inhibit flowering transition through temperature-sensitive pathways and photoperiod pathways.