作物栽培·遗传育种

硫化氢对大豆铝胁迫耐受性的增强作用

  • 刘文文 ,
  • 纪芳 ,
  • 王华华
展开
  • (河南师范大学生命科学学院,河南 新乡 453007)
刘文文(1994-),女,河南安阳人,在读硕士研究生,研究方向:植物抗逆生理。E-mail:liuwenw2017@163.com

收稿日期: 2019-06-02

  网络出版日期: 2019-10-15

基金资助

家自然科学基金项目(U1704121);河南省高校科技创新人才支持计划项目(20HASTIT038);河南师范大学优秀青年科学基金项目(14YQ003)

 The Role of Hydrogen Sulphide in Improving Aluminium Tolerance of Soybean

Expand
  •  ( College of Life Sciences,Henan Normal University,Xinxiang 453007,China)

Received date: 2019-06-02

  Online published: 2019-10-15

摘要

铝毒害是酸性土壤地区制约农作物生产的主要逆境因子。为明确作物抵抗铝毒害的机制,以大豆为试验材料,研究了新型植物气体信号分子硫化氢(H2S)在铝胁迫耐受性中的调节作用。结果显示,铝胁迫处理诱导大豆根尖H2S的产生。添加外源H2S供体处理显著缓解了铝胁迫诱导的根伸长受抑,以及根尖铝含量、根尖细胞死亡率、根尖丙二醛和活性氧(H2O2和O2·)含量升高,而添加H2S合成抑制剂处理则产生了相反的效应,加重了铝胁迫对大豆的伤害;进一步研究显示,铝胁迫下添加H2S供体处理增强了根尖抗氧化酶(APX、CAT、POD、SOD)的活性,而添加H2S合成抑制剂处理则抑制了抗氧化酶活性的增强。表明铝胁迫下H2S通过提高根尖抗氧化能力来清除活性氧的过量积累,缓解铝胁迫对根伸长的抑制。

本文引用格式

刘文文 , 纪芳 , 王华华 . 硫化氢对大豆铝胁迫耐受性的增强作用[J]. 河南农业科学, 2019 , 48(10) : 64 -69 . DOI: 10.15933/j.cnki.1004-3268.2019.10.010

Abstract

 Aluminium (Al) toxicity is a major constraint on agricultural production in acidic soils.To elucidate how crops cope with Al toxicity,the role of hydrogen sulphide (H2S) ,a novel gas signaling molecule in plants,was investigated in soybean under Al stress.Results showed that Al treatment induced H2S production in root tips.Furthermore,application of an H2S donor significantly alleviated Al-induced inhibition of root elongation,and decreased Al accumulation,cell deathrate,malondialdehyde and reactive oxygen species ( H2O2and O2) contents in root tips,whereas the treatment with an H2S inhibitor elicited the opposite effects.Further investigation showed that H2 S donor increased the activities of antioxidant enzymes (APX,CAT,POD and SOD),while the H2S inhibitor treatment inhibited the antioxidant enzymes activities in root tips under Al stress.These results indicate that H2S alleviates Al induced root elongation inhibition through enhancement of antioxidant defenses in soybean,subsequently reducing reactive oxygen species accumulation.

参考文献

[1]KOCHIAN L,PIEROS M,LIU J,et al.Plant adaptation to acid soils:The molecular basis for crop aluminum resistance[J].Annual Review of Plant Biology,2015,66:571-598.

2LIU N,SONG F,ZHU X,et al.Salicylic acid alleviates aluminum toxicity in soybean roots through modulation of reactive oxygen species metabolismJ.Frontiers in Chemistry,2017,5:96.

3GARCAMATA C,LAMATTINA L.Gasotransmitters are emerging as new guard cell signaling molecules and regulators of leaf gas exchangeJ.Plant Science,2013,201:66-73.

4SSUFFI D,ALVAREZ C,LASPINA N,et al.Hydrogen sulfide generated by Lcysteine desulfhydrase acts upstream of nitric oxide to modulate abscisic aciddependent stomatal closureJ.Plant Physiology,2014,166(4):2065-2076.

5ZHANG H,HU L Y,HU K D,et al.Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stressJ.Journal of Integrative Plant Biology,2008,50(12):1518-1529.

6ZHANG H,TANG J,LIU X P,et al.Hydrogen sulfide promotes root organogenesis in Ipomoea batatas,Salix matsudana and Glycine max[J.Journal of Integrative Plant Biology,2009,51(12):1086-1094.

7CHRISTOU A,MANGANARIS G A,PAPADOPOULOS I,et al.Hydrogen sulfide induces systemic tolerance to salinity and nonionic osmotic stress in strawberry plants through modification of reactive species biosynthesis and transcriptional regulation of multiple defence pathwaysJ.Journal of Experimental Botany,2013,64(7):1953-1966.

8HE H,LI Y,H E L F.The central role of hydrogen sulfide in plant responses to toxic metal stressJ.Ecotoxicology and Environmental Safety,2018,157:403-408.

9FANG H,LIU Z,LONG Y,et al.The Ca2+/calmodulin2binding transcription factor TGA3 elevates LCD expression and H2S production to bolster Cr6+ tolerance in ArabidopsisJ.Plant Journal,2017,91(6):1038-1050.

10PENG R,BIAN Z,ZHOU L,et al.Hydrogen sulfide enhances nitric oxideinduced tolerance of hypoxia in maize(Zea mays L.)J.Plant Cell Reports,2016,35(11):2325-2340.

11ZHU C Q,ZHANG J H,SUN L M,et al.Hydrogen sulfide alleviates aluminum toxicity via decreasing apoplast and symplast Al contents in riceJ.Frontiers in Plant Science,2018,9:294.

12]〖JP3WANG H H,ZHANG Y Y,HOU J J,et al.Nitric oxide mediates aluminuminduced citrate secretion through regulating the metabolism and transport of citrate in soybean rootsJ.Plant and Soil,2019,435(1/2):127-142.

13HE H,HUANG W,OO T L,et al.Nitric oxide suppresses aluminuminduced programmed cell death in peanut(Arachis hypoganeaL.) root tips by improving mitochondrial physiological propertiesJ.Nitric Oxide,2018,74:47-55.

14WANG H H,LI Y,HOU J J,et al.Nitrate reductasemediated nitric oxide production alleviates Alinduced inhibition of root elongation by regulating the ascorbateglutathione cycle in soybean rootsJ.Plant and Soil,2017,410(1/2):453-465.

15WANG H H,HOU J J,LI Y,et al.Nitric oxidemediated cytosolic glucose6phosphate dehydrogenase is involved in aluminum toxicity of soybean under high aluminum concentrationJ.Plant and Soil,2017,416(1/2):39-52.

16POONAM P,RAJNEESH K S,DUBEY R S.Salicylic acid alleviates aluminum toxicity in rice seedlings better than magnesium and calcium by reducing aluminum uptake,suppressing oxidative damage and increasing antioxidative defenseJ.Ecotoxicology,2013,22(4):656-670.

17HE H,HUANG W,OO T L,et al.Nitric oxide inhibits aluminuminduced programmed cell death in peanut(Arachis hypoganea L.) root tipsJ.Journal of Hazardous Materials,2017,333:285292.

18YAMAMOTO Y,KOBAYSHI H.Lipid peroxidation is an early symptom triggered by aluminum,but not the primary cause of elongation inhibition in pea rootsJ.Plant Physiology,2001,125(1):199-208.

19CRDOBAPEDREGOSA M C,CRDOBA F,VILLALBA J M,et al.Changes in intracellular and apoplastic peroxidase activity,ascorbate redox status,and root elongation induced by enhanced ascorbate content in Allium cepa LJ.Journal of Experimental Botany,2005,56(412):685-694.

20GRAT O P L,MONTEIRO C C,TEZOTTO T,et al.Cadmium stress antioxidant responses and root to shoot communication in grafted tomato plantsJ.Biometals,2015,28(5):803-816.

21LIU X,CHEN J,WANG G H,et al.Hydrogen sulfide alleviates zinc toxicity by reducing zinc uptake and regulating genes expression of antioxidative enzymes and metallothioneins in roots of the cadmium/zinc hyperaccumulator Solanum nigrumL.J.Plant and Soil,2016,400(1/2):177-192.

22ZHANG H,HU L Y,LI P,et al.Hydrogen sulfide alleviated chromium toxicity in wheatJ.Biologia Plantarum,2010,54(4):743-747.

 

文章导航

/