驱蚊酯长效驱避剂的制备及其含量测定

doi:10.15933/j.cnki.1004-3268.2021.02.020

河南农业科学 ›› 2021, Vol. 50 ›› Issue (2): 162-166.DOI: 10.15933/j.cnki.1004-3268.2021.02.020

驱蚊酯长效驱避剂的制备及其含量测定

赵聪¹，岳治光²，黄斌²，陈倩²，张素梅¹，菅复春¹，张龙现¹，王荣军¹，宁长申¹

(1.河南农业大学牧医工程学院，河南郑州 450002;2.河南安进生物技术股份有限公司，河南郑州 410105)

收稿日期:2020-08-06 出版日期:2021-02-15 发布日期:2021-02-15
通讯作者: 宁长申(1958-),男,河南郾城人,教授,主要从事兽医寄生虫学与寄生虫病防控研究。E-mail:nnl1986@163.com
作者简介:赵聪(1996-),男,河南兰考人,在读硕士研究生,研究方向：动物寄生虫病防控。E-mail:1519953894@qq.com
基金资助:
国家重点研发计划项目（2018YFD0502100）;国家现代肉羊产业技术体系项目（nycytx-38）

Preparation and Content Determination of IR3535 Long-Term Repellent

ZHAO Cong¹,YUE Zhiguang²,HUANG Bin²,CHEN Qian²,ZHANG Sumei¹,JIAN Fuchun¹,ZHANG Longxian¹,WANG Rongjun¹,NING Changshen¹

(1.College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China;2.Henan Amgen Biotechnology Co.,Ltd.,Zhengzhou 410105,China)

Received:2020-08-06 Published:2021-02-15 Online:2021-02-15

摘要/Abstract

摘要： 为研制长久持效的硬蜱驱避剂，依据驱蚊酯的理化特性，以驱蚊酯为有效成分、无水乙醇为溶剂、α-吡咯烷酮为稳定剂、聚乙二醇-400（PEG-400）为增黏剂，分别以聚乙烯吡咯烷酮（PVP-K30）、羧甲基纤维素钠(CMC-Na)、麦芽糊精为缓释成分进行多种溶剂系统组合，设计4个驱蚊酯含量为20%的驱避剂原药配方，并进行系列稳定性试验，对配方进行筛选，并对筛选出的配方进行相关含量测定。结果表明，采用5%～20%的聚乙烯吡咯烷酮、5%～20%的α-吡咯烷酮、5%～20%的聚乙二醇-400、30%的无水乙醇为组成成分的原药配方具有最好的稳定性。利用气相色谱法测定制剂中驱蚊酯的含量，所制备的驱蚊酯长效驱避剂的平均日内精密度为0.59%，平均日间精密度为0.49%，平均回收率为97.4%，3个批次的驱蚊酯长效驱避剂含量均介于标示量的98.1%～103.8%。综上，制备的驱蚊酯长效驱避剂工艺简单，稳定性好，可重复性高。

关键词: 驱蚊酯, 硬蜱, 含量测定, 长效驱避剂, 气相色谱法

Abstract: In order to develop long-lasting repellent for ticks, four repellent formulations with 20% IR3535 content were designed. According to the physical and chemical characteristics of IR3535,with IR3535 as active component, absolute ethanol as solvent, α-pyrrolidone as stabilizer, PEG-400 as tackifier,PVP-K30, CMC-Na and maltodextrin as slow-release components combined in a variety of solvent systems to design.The results of stability test showed that the solvent formulation with 5%—20%PVP,5%—20% α-pyrrolidone,5%—20% PEG-400 and 30% anhydrous ethanol had the best stability.The content of mosquito repellent was determined by gas chromatography.The results showed that the average intra day precision was 0. 59%,the average inter day precision were 0.49%,and the average recovery was 97.4%.The content of three batches of long-acting repellent was between 98.1% and 103.8% of the labeled amount.To sum up,the preparation of IR3535 long-acting tick repellent agent is simple,stable and repeatable.

Key words: IR3535, Tick, Content determination, Long-term repellent, Gas chromatography

中图分类号:

S859.79

赵聪, 岳治光, 黄斌, 陈倩, 张素梅, 菅复春, 张龙现, 王荣军, 宁长申. 驱蚊酯长效驱避剂的制备及其含量测定[J]. 河南农业科学, 2021, 50(2): 162-166.

ZHAO Cong, YUE Zhiguang, HUANG Bin, CHEN Qian, ZHANG Sumei, JIAN Fuchun, ZHANG Longxian, WANG Rongjun, NING Changshen. Preparation and Content Determination of IR3535 Long-Term Repellent[J]. Journal of Henan Agricultural Sciences, 2021, 50(2): 162-166.

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驱蚊酯长效驱避剂的制备及其含量测定

Preparation and Content Determination of IR3535 Long-Term Repellent

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