中甸犏牛SLC11A1基因3′非翻译区多态位点研究及其与抗病性关联的评估

河南农业科学 ›› 2018, Vol. 47 ›› Issue (9): 131-137.

中甸犏牛SLC11A1基因3′非翻译区多态位点研究及其与抗病性关联的评估

王利平^1,2，席冬梅¹，熊和丽¹，李国治¹，李鹏²，邓卫东^1*

（1.云南农业大学动物科学技术学院，云南昆明 650201； 2.新乡学院生命科学与技术学院，河南新乡 453000）

收稿日期:2018-04-01 出版日期:2018-09-15 发布日期:2018-09-15
通讯作者: 邓卫东（1972-），男，陕西石泉人，教授，博士，主要从事动物遗传资源开发利用与评价。E-mail:1692425306@qq.com
作者简介:王利平（1980-），女，河南临颍人，讲师，博士，主要从事动物遗传资源开发利用与评价。E-mail:93612020@qq.com
基金资助:
国家自然科学基金项目（31460583）；云南省现代肉牛产业技术体系项目（2017KJTX0015）

Received:2018-04-01 Published:2018-09-15 Online:2018-09-15

摘要/Abstract

摘要： 为研究中甸犏牛溶质载体转运蛋白基因（SLC11A1）的结构变异情况，并评估结构变异与抗病性的关联，采集了114个样品，扩增了SLC11A1基因3′非翻译区（3′UTR）部分序列，测序共检测到3个微卫星多态位点（MS1、MS2、MS3）和3个核苷酸变异（1 782位点T→G、1 805位点G→A、1 907位点G→T），其中MS1有6个等位基因（GT10、GT12、GT14—17）、MS2有3个等位基因（GT5—7）、MS3有5个等位基因（GT12—16）。GT12/15、GT5/5、GT13/13基因型频率最高，分别为0.204、0.05、0.324；GT15、GT5、GT13等位基因频率最高分别为0.284、0.685、0.433。对MS1、MS2、MS3这3个多态位点进行单倍型分析，发现了44种单倍型，其中GT12/5/13为优势单倍型，频率为0.13。χ2比较分析表明，中甸犏牛与婆罗门瘤牛×布兰科牛、荷斯坦牛×布兰科牛、布兰科牛、非洲牛MS1位点的GT12/12基因型频率差异显著（P<0.01），与西班牙黄牛、荷兰黄牛MS3的GT13/13基因型频率差异显著（P<0.01），与非洲黄牛差异不显著（P>0.05）。综上，中甸犏牛SLC11A1基因3′UTR具有丰富的多态性，推断其GT12/12和GT13/13纯合子基因型与抗病性相关。

王利平，席冬梅，熊和丽，李国治，李鹏，邓卫东. 中甸犏牛SLC11A1基因3′非翻译区多态位点研究及其与抗病性关联的评估[J]. 河南农业科学, 2018, 47(9): 131-137.

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