耐寡营养高效解磷菌株XMT-5的分离鉴定及解磷特性

河南农业科学 ›› 2017, Vol. 46 ›› Issue (8): 67-71.

耐寡营养高效解磷菌株XMT-5的分离鉴定及解磷特性

李海峰¹，张月阳¹，曹健²，屈建航¹，田海龙¹

1.河南工业大学生物工程学院，河南郑州 450001； 2.中原工学院，河南郑州 450007

收稿日期:2017-02-20 出版日期:2017-08-15 发布日期:2017-08-15
作者简介:李海峰(1984-)，女，河北涿鹿人，副教授，博士，主要从事微生物与磷循环研究。E-mail:hfli@haut.edu.cn
基金资助:
国家自然科学基金项目(31400103,31370147)；河南省高校科技创新团队支持计划项目（15IRTSTHN019）

Received:2017-02-20 Published:2017-08-15 Online:2017-08-15

摘要/Abstract

摘要： 为获得更加适应寡营养的自然环境条件的解磷菌株，以1/4蒙金娜培养基从土壤中分离筛选高效解磷菌株，并对其进行鉴定，分析其生长条件及解磷机制。结果表明，从土壤中共分离获得解磷菌9株，通过对解磷量的定量测定确定高效解磷菌株XMT-5，其最大解磷量为195.63 mg/L；从菌落特征、生理生化特性及16S rRNA基因序列分析三方面鉴定菌株XMT-5为根瘤菌（Rhizobium sp.）；菌株XMT-5在寡营养条件下可以分泌柠檬酸、酒石酸和乙酸3种有机酸，从而可使培养液pH值下降；菌株XMT-5可在温度4～42 ℃、pH值4～10、NaCl质量浓度0～80 g/L以及寡营养条件下生长，环境适应能力较强，具有良好的应用前景。

关键词: 寡营养, 解磷菌, 解磷特性, 生物磷肥

李海峰，张月阳，曹健，屈建航，田海龙. 耐寡营养高效解磷菌株XMT-5的分离鉴定及解磷特性[J]. 河南农业科学, 2017, 46(8): 67-71.

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