虹鳟hif-1a 基因家族的拷贝数变异及分歧进化

doi:10.15933/j.cnki.1004-3268.2021.11.018

河南农业科学 ›› 2021, Vol. 50 ›› Issue (11): 153-161.DOI: 10.15933/j.cnki.1004-3268.2021.11.018

虹鳟hif-1a 基因家族的拷贝数变异及分歧进化

王宪宗¹，宋晶¹，杨春娟²

（1.山西农业大学动物科学学院，山西太谷 030801；2.山西省水产技术推广站，山西太原 030002）

收稿日期:2021-07-12 出版日期:2021-11-15 发布日期:2022-01-18
作者简介:王宪宗（1987-），男，山东济宁人，讲师，博士，主要从事鱼类分子进化与遗传资源研究。E-mail：xianzong_wang@126.com
基金资助:
山西省高等学校科技创新项目（2020L0158）；山西农业大学科技创新项目（2017YJ05）

Copy Number Variation and Divergence of hif-1a Gene Family in Oncorhynchus mykiss

WANG Xianzong¹，SONG Jing¹，YANG Chunjuan²

（1.College of Animal Science，Shanxi Agricultural University，Taigu 030801，China；2.Station for Popularizing Aquatic Technology of Shanxi Province，Taiyuan 030002，China）

Received:2021-07-12 Published:2021-11-15 Online:2022-01-18

摘要/Abstract

摘要： 为深入阐明虹鳟不耐低氧的分子机制，采用BLAST搜索、重构最大似然树来确定其hif-1a 家族的拷贝数，并对这些拷贝的组织表达谱、共表达基因及其所富集的GO条目、编码蛋白质的保守结构域分布及部分结构域的三维结构进行比较。结果显示，虹鳟的hif-1a 有2个拷贝，与它们直系同源的斑马鱼基因均是hif-1aa。2个hif-1a 拷贝的组织表达谱存在显著的分化，它们的共表达基因数量及所富集的与血管生成、细胞增殖、物质运输和代谢相关的GO条目数量也存在较大差异；它们所编码的蛋白质均拥有5个保守结构域，C-TAD结构域三维结构均存在结构创新，但bHLH结构域的三维结构存在较大差异。综上，虹鳟在较近的全基因组重复事件之前丢失了hif-1ab，现有的2个hif-1aa 拷贝在朝亚功能化方向快速进化，某些地理种群中可能已经存在有利于提高耐低氧性能的基因型。

关键词: 虹鳟, hif-1a 基因家族, 低氧, 全基因组重复, 共表达基因, 亚功能化

Abstract: To further elucidate the molecular mechanism of hypoxia intolerance of Oncorhynchus mykiss，BLAST search and reconstruction of maximum likelihood tree were conducted to determine the copy number of hif-1a family.Tissue expression profiles，co‑expressed genes and their enriched GO terms，distribution of conserved domains and three‑dimensional structures of partial domains were compared between these copies.The results showed that there were two copies of hif-1a in O.mykiss，the orthologs of which in Danio rerio were all hif-1aa.The two copies varied significantly in tissue expression profiles.Their co‑expressed genes and GO terms related to angiogenesis，cell proliferation，material transport and metabolism enriched by these co‑expressed genes also varied greatly in number.The proteins encoded by them both contained five conserved domains，among which the two C‑TAD domains both contained structural innovation，while the two bHLH domains varied greatly in three‑dimensional structure.Collectively，O.mykiss lost hif-1ab before the recent whole genome duplication event，while the two existing hif-1aa copies have been rapidly evolving towards subfunctionalization.Some geographical
populations might already have genotypes that are helpful to improve hypoxia tolerance.

Key words: Oncorhynchus mykiss, hif?1a gene family, Hypoxia, Whole genome duplication, Co?expressed gene, Subfunctionalization

中图分类号:

S917.4

王宪宗, 宋晶, 杨春娟. 虹鳟hif-1a 基因家族的拷贝数变异及分歧进化[J]. 河南农业科学, 2021, 50(11): 153-161.

WANG Xianzong, SONG Jing, YANG Chunjuan. Copy Number Variation and Divergence of hif-1a Gene Family in Oncorhynchus mykiss[J]. Journal of Henan Agricultural Sciences, 2021, 50(11): 153-161.

图/表 10

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虹鳟hif-1a 基因家族的拷贝数变异及分歧进化

Copy Number Variation and Divergence of hif-1a Gene Family in Oncorhynchus mykiss

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