牛支原体iTRAQ定量蛋白质组学分析

doi:10.15933/j.cnki.1004-3268.2021.10.018

摘要/Abstract

摘要： 为了解牛肺炎支原体临床分离菌株（HN12）与标准菌株（PG45）蛋白质差异表达情况，采用定量蛋白质组学研究策略，在牛支原体临床分离菌株（HN12）和标准菌株（PG45）的全蛋白质中进行差异蛋白质鉴定，并进行生物信息学分析。结果表明，共鉴定2 402个蛋白质，其中存在显著差异的有66个。上调蛋白质数量为25个，下调蛋白质数量为41个。鉴定出的蛋白质主要有膜蛋白、跨膜蛋白、脂蛋白、延长因子、膜脂蛋白P81、伴侣蛋白、可变表面脂蛋白和ABC转运蛋白等。

关键词: 牛支原体肺炎, 牛支原体, 定量蛋白质组学, 差异蛋白质

Abstract: To understand the differentially expressed proteins‑Bovine Mycoplasma bovis clinical strain（HN12）and standard strain（PG45），this study adopted the quantitative proteomics research strategies，to identify differentially expressed proteins in total proteins of the clinical strain（HN12）and standard strain（PG45）and carried out bioinformatics analysis. The results showed as follows：a total of 2 402 proteins were identified，of which 66 were significantly different. Among them，the numbers of up‑regulated and down‑regulated proteins were 25 and 41 respectively. Proteins identified mainly included membrane protein，transmembrane protein，lipoprotein，prolongation factor，membrane lipoprotein P81，chaperone，variable surface lipoprotein，and ABC transporter，et al.

Key words: Bovine Mycoplasma pneumonia, Mycoplasma bovis, Quantitative proteomics, Differential protein

中图分类号:

S855

李海利, 徐引弟, 游一, 朱文豪, 张青娴, 焦文强, 王治方, 许峰, 王克领, 郎利敏, 张立宪, 邹靖. 牛支原体iTRAQ定量蛋白质组学分析[J]. 河南农业科学, 2021, 50(10): 138-145.

LI Haili, XU Yindi, YOU Yi, ZHU Wenhao, ZHANG Qingxian, JIAO Wenqiang, WANG Zhifang, XU Feng, WANG Keling, LANG Limin, ZHANG Lixian, ZOU Jing. iTRAQ Quantitative Proteomic Analysis of Mycoplasma bovis[J]. Journal of Henan Agricultural Sciences, 2021, 50(10): 138-145.

图/表 8

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