关键词: 鼠尾草, 花粉形态, 扫描电镜, 物种进化, 亲缘关系

Abstract: In order to clarify the pollen morphological characteristics of some ornamental plants of the Salvia L.genus, and explore the degree of evolution and the relationship between them, the pollen morphology of 5 species (Salvia microphylla‘Hot Lips’,Salvia leucantha,Salvia greggii,Salvia elegans and Salvia splendens‘Vista Purple’) of Salvia L.was observed by scanning electron microscopy and according to the principal component scores of polar axis length, equatorial axis length and germinal groove width cluster analysis was conducted.The results showed that the pollen of five species of Salvia L.was single-grain,the polar axis length of pollen was 65.70—120.71 μm,the range was large, the largest pollen grain was Salvia splendens‘Vista Purple’,and the smallest was Salvia leucantha.The P/E values of five pollens were between 1.15 and 1.23,all of which belonged to oblong pollens.The pollen of the genus Salvia L. had 6 germination grooves,and the outer wall was decorated with large and small meshes or three sets of reticulation,and the shape of mesh was mostly irregular polygon.The ridges were thick.According to the evolution rule of pollen surface decoration and pollen volume size,Salvia splendens ‘Vista Purple’ was far from the other four species,and the degree of evolution was high,while Salvia leucantha and other species were more primitive species. At the genetic distance of 2.0,5 species of Salvia L.were divided into three groups.Salvia microphylla‘Hot Lips’,Salvia elegans and Salvia greggii were divided into one group,while Salvia leucantha and Salvia splendens‘Vista Purple’were separately divided into one group.

Key words: Salvia L., Pollen morphology, Scanning electron microscopy, Species evolution, Genetic relationship