关键词: 石苦秦散复方制剂, 鸭疫里氏杆菌, 多杀性巴氏杆菌, MIC, MBC

Abstract: In order to study the antibacterial activity in vitro of Shikuqin powder and its main active component monomers on waterfowl-origin multidrug-resistant strains,in this study,K-B method was used to detect the antibacterial agent resistance of the tested strains. Double dilution method of micro broth and agar plate culture counting method were used to investigate the antibacterial activity or bactericidal activity of Shikuqin powder and its main active component monomers on Riemerella anatipestifer,Escherichia coli,Salmonella,Pasteurella multocida and Staphylococcus aureus.The results showed that 90% of the isolates were multi-drug resistant strains.Escherichia coli and Salmonella isolates of Enterobacter were the most antibiotic resistant strains,and the number of antibiotic-resistant strains was higher than 8 kinds.The MICs of Shikuqin powder for five kinds of bacteria ranged from 1.95 g/L to 62.50 g/L,and MBCs ranged from 1.95 g/L to 125.00 g/L.The MICs of Shikuqin powder for Riemerella anatipestifer and Pasteurella multocida were lower,ranging from 1.95 g/L to 15.63 g/L.Among the six Chinese herbal monomers,esculetin was the strongest,followed by matrine and gallic acid.The bactericidal efficacy of the six Chinese herbal monomers varied greatly.When the concentration reached 31.25 g/L,gallic acid could kill all the tested strains,and the overall bactericidal efficacy was better than the other five monomers.The MIC and MBC of the six Chinese herbal monomers against Riemerella anatipestifer were lower than those of other strains.It can be seen that Shikuqin powder has antibacterial effect on these five kinds of bacteria,among which the bacteriostasis effect on Riemerella anatipestifer and Pasteurella multocida is the best.The bacteriostatic and bactericidal effects of six Chinese herbal monomers on Riemerella anatipestifer were superior to those of other strains.

Key words: Shikuqin powder, Riemerella anatipestifer, Pasteurella multocida; MIC, MBC