Abstract: In order to investigate the effect of polymorphism of the first exon of OC-116 gene on eggshell quality,Changshun Blue-eggshell chicken was used as materials and the single nucleotide polymorphism(SNP) in the first exon region of OC-116 gene was detected by the direct sequencing of PCR products.General linear model was used to analyzed the genetic effects of polymorphic loci on shell quality of Changshun Blue-eggshell chicken. The results showed that two moderately polymorphic and fully linked SNPs were detected in exon 1 of the OC-116 gene,g.45667632 T>C and g.45667671 G>A,respectively,and three genotypes were generated.Chi-square(χ2) test found that the genotype distribution of the two SNPs did not deviate from the Hardy-Weinberg equilibrium(P>0.05),and there were two haplotypes and three diplotypes in the two SNPs.The genotypes CC,AA and diplotype H2H2 were significantly higher in egg quality than genotypes TT,GG and diplotype H1H1 (P<0.05);the genotypes TT,GG and diplotype H1H1 were significantly higher in egg shape index than genotypes CT,AG and diplotype H1H2 (P<0.05);the genotypes CT,AG and diplotype H1H2 were significantly higher in eggshell strength than genotypes TT,GG and diplotype H1H1(P<0.05).Taken together,genotypes CC,AA and diplotype H2H2 are favorable genotypes and diplotype for improving eggshell quality,revealing that g. 45667632 T>C site and g.45667671 G>A site may serve as marker sites for shell trait selection.

Key words: Changshun Blue-eggshell chicken, OC-116 gene, Exon, Eggshell quality, SNP

摘要： 为探讨OC-116基因第1外显子多态性对鸡蛋壳品质的影响，试验以长顺绿壳蛋鸡为材料，采用PCR产物直接测序法对OC-116基因第1外显子区域进行单核苷酸多态性(SNP)筛查，利用一般线性模型分析多态位点对长顺绿壳蛋鸡蛋壳品质的遗传效应。结果显示,在OC-116基因第1外显子共检测到2个中度多态且完全连锁的SNP位点，分别为g.45667632 T>C和g.45667671 G>A，均产生3种基因型。通过卡方（χ²）检验发现，2个SNPs基因型分布均未偏离Hardy-Weinberg平衡（P>0.05），共存在2种单倍型和3种双倍型。基因型CC、AA和双倍型H2H2在蛋质量上显著高于基因型TT、GG和双倍型H1H1（P<0.05）,基因型TT、GG和双倍型H1H1在蛋形指数上显著高于基因型CT、AG和双倍型H1H2（P<0.05），基因型CT、AG和双倍型H1H2在蛋壳强度上显著高于基因型TT、GG和双倍型H1H1（P<0.05）。综上，基因型CC、AA和双倍型H2H2是改善蛋壳品质的有利基因型和双倍型，揭示g.45667632 T>C位点和g.45667671 G>A位点可作为蛋壳性状选择的标记性位点。

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