Plant Resistance to Leaf Rust in 50 Foreign Wheat Cultivars

doi:10.15933/j.cnki.1004-3268.2019.12.012

Abstract

Abstract: A total of 50 introduced wheat cultivars were used for identifying seedling resistance gene for leaf rust at seedling stage and slow rusting resistance at adult stage. At seedling stage 20 Puccinia triticina pathotypes with virulent genes were used to inoculate the 50 introduced cultivars and 36 differential cultivars with known leaf rust resistance genes for postulating seedling genes present in these cultivars. Molecular marker were also used to test the cultivars for further confirming the results based on gene postulation.These cultivars were also tested for slow rusting resistance in field in two consecutive years at two locations.The results based on gene postulation and molecular marker detection showed that each of Lr1 and Lr26 were identified in five cultivars. Insijnia,Palpich,MV laura,Mason/jagger and Re7145 contained Lr1,while Lr26 were found in Insijnia,Tx03a0148,F98047j14-2inc,T67/X84w063-9-45// K92 and Mason/jagger.Based on gene postulation and molecular marker,Lr34 were detected in 13 cultivars(Hk1/6/Nvsr3/5bez/tvr,Tx03a0148,Palpich,Kanto107,MV laura,F92080g1-1/F93042g2-1,Mv05-08,Norin61,Bruta,Aca801,F98047j14-2inc,T67/X84w063-9-45//K92 and Mason/jagger)and Lr37 were present in 11 cultivars(Nidera baguette 10,Insijnia,Nsa09-3645,Soissons,Aztec,Carimulti,Mason/jagger,Re714,Kniish-46,Nuwest/4/D887-74/pew/and Fr03733).Lr46 was detected in 45 cultivars(Sagittario,Hk1/6/Nvsr3/5bez/tvr,Insijnia,Fr03717,Dorico etc.).Lr18 was present in two cultivars(T67/X84w063-9-45//K92 and Re7145) and three cultivars (Fr03724,Fr3713 and T67/X84w063-9-45//K92) contained Lr21.Lr36 was found only in T67/X84w063-9-45//K92.19cultivars(Mv05-08, Fr03725,Re714,Fr03717,Fr03724 etc.) slowed rusting resistance in the field trials.

Key words: Foreign wheat germplasm, Leaf rust, Gene postulation, Seedling stage assay, Adult stage identification;Leaf rust resistance gene

摘要： 为确定小麦品种（系）所携带的抗病基因，对来自国外的50个小麦品种（系）进行苗期抗叶锈病基因推导和成株期抗叶锈鉴定。在苗期，根据供试品种（系）与20个毒力不同的叶锈菌生理小种的互作反应，与36个已知抗叶锈基因载体品种的侵染型进行比较，同时对试验材料进行连续2a两点的田间抗锈性试验，并利用分子标记进一步检测供试品种（系）中含有的抗叶锈病基因。基因推导和标记检测结果表明，Insijnia、Palpich、MV laura、Mason/jagger、Re7145共5个小麦品种（系）含有Lr1，Insijnia、Tx03a0148、F98047j14-2inc、T67/X84w063-9-45//K92、Mason/jagger共5个小麦品种（系）含有Lr26，Hk1/6/Nvsr3/5bez/tvr、Tx03a0148、Palpich、Kanto107、MV laura、F92080g1-1/F93042g2-1、Mv05-08、Norin61、Bruta、Aca801、F98047j14-2inc、T67/X84w063-9-45//K92、Mason/jagger共13个小麦品种（系）含有成株慢锈基因Lr34，Nidera baguette 10、Insijnia、Nsa09-3645、Soissons、Aztec、Carimulti、Mason/jagger、Re714、Kniish-46、Nuwest/4/D887-74/pew/、Fr03733共11个小麦品种（系）含有成株抗性基因Lr7，Sagittario、Hk1/6/Nvsr3/5bez/tvr、Insijnia、Fr03717、Dorico 等共45个品种（系）含有成株慢锈基因Lr46，T67/X84w063-9-45//K92、Re7145共2个品种（系）可能含有Lr18，Fr03724、Fr3713、T67/X84w063-9-45//K92共3个品种（系）可能含有Lr21，T67/X84w063-9-45//K92仅1个品种（系）可能含有Lr36。田间试验结果表明，Mv05-08、Fr03725、Re714、Fr03717、Fr03724等共19个品种（系）表现慢锈性。

关键词: 国外小麦种质, 小麦叶锈病, 基因推导, 苗期鉴定, 成株期鉴定, 抗叶锈基因

CLC Number:

S435

JIAO Yue, WANG Siman, ZHAO Xilan, ZHANG Peipei, ZHENG Huimin, LI Zaifeng, LIU Daqun. Plant Resistance to Leaf Rust in 50 Foreign Wheat Cultivars[J]. Journal of Henan Agricultural Sciences, 2019, 48(12): 79-88.

焦悦, 王思曼, 赵喜兰, 张培培, 郑慧敏, 李在峰, 刘大群. 50个国外小麦品种（系）抗叶锈性鉴定[J]. 河南农业科学, 2019, 48(12): 79-88.

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