In order to establish the technology of rapid fungicides selection against Colletotrichum gloeosporioides,100 isolates of C.gloeosporioides were isolated and collected from Guizhou.The sensitivity baslines of C.gloeosporioides to thiophanate methyl and prochloraz were determined by mycelial growth rate method.Based on sensitivity basline of C.gloeosporioides to thiophanate methyl and prochloraz,the rapid fungicides selection kit against C.gloeosporioides was established using bromocresol purple.The results showed that the EC₅₀ mean values of (0.7641±0.1508)μg/mL and (0.0710±0.0133)μg/mL were sensitivity baslines of C.gloeosporioides to thiophanate methyl,prochloraz in Guizhou region,respectively.When the strain of C.gloeosporioides was placed in selection kit of thiophanate methyl of 0.45 μg/mL,the color of the kit was orange red,indicating that the strain of C.gloeosporioides to thiophanate methyl was sensitive.But when the strain of C.gloeosporioides was placed in selection kit of thiophanate methyl of 1.80 μg/mL,the color of the kit was yellow,indicating that the strain was resistant.When the strain of C.gloeosporioides was placed in selection kit of prochloraz of 0.088 μg/mL,the color of the kit was orange red,indicating that the strain of C.gloeosporioides to prochloraz was sensitive.But when the strain of C.gloeosporioides was placed in selection kit of prochloraz of 0.350 μg/mL,the color of the kit was yellow,indicating that the strain was resistant.