In order to screen out the light transmittance and 24-epibrassinolide (EBR) concentration suitable for triennial Panax notoginseng growth, shading network was used to set five different light transmittance(57.31%,31.61%,18.90%,11.44%,8.04%) and six concentrations of EBR solution[0(water),0.05, 0.20, 0.40, 0.80, 1.60 mg/L] was set up to explore the effect of different light transmittance and different concentrations of EBR solution on agronomic characteristics,quality and yield of triennial Panax notoginseng in field experiment.The results showed that the morphologic indexes of Panax notoginseng were different under different treatments,when the light transmittal rate was 18.90%,the plant height,middle leaf length,middle leaf width and fresh weight per plant of Panax notoginseng were significantly higher than those under other treatments,which were 33.67 cm,14.06 cm,4.67 cm,53.86 g,and total saponins content was high(8.14%).The effects of different transmittance on water content,ash content,extract and total saponins contents of Panax notoginseng were not significant.When the concentration of EBR was 0.05 mg/L,the plant height,root-shoot ratio,water content and ash content of Panax notoginseng were the highest,which were 32.41 cm,2.15,6.50% and 5.22%,respectively.When EBR concentration was 0.20 and 0.40 mg/L,petiole length and relative chlorophyll content of Panax notoginseng were the highest, which were 10.36 cm and 47.21, respectively.When the concentration of EBR was 0.80 mg/L,the yield of ginseng Rg1 and individual plant was the highest (4.00% and 46.98 g,respectively),and the total saponin content was higher(7.30%).In conclusion,when the transmittance of Panax notoginseng was 18.90%,which is more suitable for the accumulation of underground biomass and saponins. When the concentration of EBR was 0.80 mg/L,the quality and yield of Panax notoginseng were the best.