To seek out the bacteria which had antifungal ability for walnut fungal disease, plate face-off test and co-culture test were carried out for antifungal characteristics of strain 7-3 in the lab.The morphological,physiological and biochemical characteristics test,and 16S rRNA 16S rRNA phylogeny analysis of strain 7-3 were studied.The results showed that strain 7-3 was Bacillus safensis 7-3,it had a certain inhibitory effect on a variety of walnut pathogens, with a broad antibacterial spectrum and strong antibacterial ability.The highest antagonistic activity was showed on the Diaporthe nobilis,inhibition rate was 82.9%.Meantime,the spore malformation of Pestalotiopsis disseminata was found when it was adhered with cell of strain 7-3,the same result was found when it was deal with high temperature sterilized fermentation broth or fermentation broth filter of strain 7-3.The results suggested that B.safensis 7-3 had excellent,stable and wide antifungal ability,its potential application was presented as a biological control agent for walnut fungal diseases management.