In order to develop ASFV immunodiagnostic reagents,recombinant P30 protein of ASFV was prepared by Escherichia coli expression system.Monoclonal antibodies were prepared and identified to establish a gold immunochromatographic assay(GICA)for the detection of ASFV.The results showed that the recombinant expression plasmid pET28a-P30 was constructed successfully. Soluble recombinant P30 protein was expressed,and the protein could react with ASFV positive serum.Ten positive hybridoma cells stably secreting monoclonal antibodies against ASFV protein P30 were screened.The ascites ELISA titers of 10 hybridoma cells were higher than 1 ∶ 40 000.These antibodies had no cross-reaction with other common swine viruses,and all of the 10 antibodies could react with ASFV.The obtained antibodies were used to establish a GICA,which could be used to detect ASFV.