To study biological function of arginine decarboxylase gene(ADC) in growth and development of peach tree,the line overexpression the PpADC gene was used to analysis how ADC affected the growth of tomato, the growth and development status of transgenic plant was observed, and the influence of exogenous gibberellin on the growth and development of transgenic plant was analyzed.The results showed that the PpADC gene was overexpressed in transgenic tomatoes,the contents of putrescine in transgenic lines#21and#58 were 25.54% and 30.17% higher than that in wild type tomato,respectively,and both dwarfism and late-flowering were shown in these plants with increased PpADC transcript.Gene expression analysis showed that the expression levels of gene GA20ox1 and GA3ox1 in transgenic plants were significantly lower than that of wild-type tomato,and the height of transgenic plants could be rescued by application of gibberellin,but not the development process of transgenic plants.It can be seen that PpADC gene may down-regulate the synthesis of gibberellin,and leads to dwarfism and developmental retardation,by promoting the synthesis of putrescine in plants.
WANG Baoquan, ZHANG Xiaona, LI Guohuai
. Overexpression of PpADC Gene in Tomato Induces Dwarfism and Late-flowering[J]. Journal of Henan Agricultural Sciences, 2020
, 49(10)
: 101
-107
.
DOI: 10.15933/j.cnki.1004-3268.2020.10.014