In order to clarify the mitigation effect of different control measures on continuous cropping obstacle of sorghum,three treatments which were continuous sorghum cropping treatment(T1),applying biological agent on continuous sorghum cropping(T2),sorghum/corn rotation(T3) were conducted using pot experiment, and the effects of different control measures on the growth, microbial functional diversity and Shannon index of bacteria and fungi in sorghum rhizosphere soil were studied.The results showed that applying biological agent and sorghum/corn rotation could promote the growth of sorghum.The plant height,stem diameter and biomass of sorghum with biological agent increased by 16.95%,13.33% and 19.82% compared with continuous cropping treatment,respectively.The stem diameter and biomass of sorghum/corn rotation treatment increased by 12.00% and 16.22% compared with continuous cropping treatment,respectively.The utilization of carbon sources by the sorghum rhizosphere soil microbial community showed that,the average well color development(AWCD) value of sorghum/corn rotation treatment was the highest,which was 16. 72% higher than that of continuous cropping treatment and 19.15% higher than that of applying biological agent treatment,at 96 h after culture,respectively.The result indicated that applying biological agent and sorghum/corn rotation both increased the carbon source utilization capacity of sorghum rhizosphere soil microbial community.The DGGE maps of bacteria and fungi were analyzed,the results showed that there were abundant bacterial bands in the rhizosphere soil of sorghum,and the number and brightness of bands varied among different treatments. Shannon index of rhizosphere soil bacterial community of sorghum/corn rotation treatment was significantly higher than that of continuous cropping treatment.There was an obvious difference in the number of bands of fungus 18S.Specific bands were found in continuous cropping and applying biological agent treatments,indicating that the fungi gene Shannon index in 18S zone was higher in the rhizosphere soil of continuous sorghum.Applying biological agent and sorghum/corn rotation could alleviate the continuous cropping obstacles of sorghum to a certain extent, it should be noted that the effect of applying biological agent to continuous cropping soil had the best effect.