Tobacco secretion of long-stalk secretory glandular trichomes has an important influence on the quality of tobacco leaves.In order to clarify the genetic characteristics of long-stalk glandular trichomes,we used the homozygous long-stalk secretory glandular trichome type T.I.1068 as the male parent and non-secretory glandular trichomes type T.I.1112 as the female parent to construct a population of 6 generations(P₁,P₂,F₁,F₂,BC₁and BC₂).The joint segregation analysis method of mixed major gene plus polygene genetic model was used to study the genetic characteristics of secretory and non-secretory types of long-stalk glandular trichomes.The results showed that the inheritance of the relative proportion of tobacco long-stalk secretory glandular trichomes to non-secretory glandular trichomes was controlled by two pairs of additive-dominant-epistatic major genes plus additive-dominant-epistatic-polygenic models.The heritability of the main genes of the BC₁,BC₂ and F₂ generations were 80.05%,97.88% and 96.50%,respectively.The heritability of the main genes was very high,and the selection of glandular trichomes types could be finished in the early generation; the multi-gene heritability of the BC₁,BC₂ and F₂ generation were 16.15%,1.55% and 2.75%,respectively.The impact of polygenes should be considered in the BC₁generation,while the impact of the polygenes in the BC₂and F₂ generations was negligible.Our results showed that the relative proportion of long-stalk secretory and non-secretory glandular trichomes is controlled by two pairs of main genes,mainly by major gene effects,while affected by the interaction between genes.