In order to strengthen protection,utilization and understand genetic diversity of wild germplasm of Staphylea bumalda DC in Hong’an county,24 wild germplasms of Staphylea bumalda DC were collected in different sites. Genetic diversity was studied using 21 pairs of polymorphic SSR primers,and cluster analysis was done.The results showed that a total of 56 allelic variations were detected by 21 pairs of SSR primers,and 2.95 allelic variations were detected for each pair of primers;effective alleles number(Ne)ranged from 0.635 4 to 2.748 2,and the average number was 1.622 3;Shannon-Weaver diversity index
(I) ranged from 0.296 3 to 1.731 9,and the average was 0.772 9;Nei’s genetic diversity index(H)ranged from 0.157 4 to 0.762 5,and the average was 0.361 8;polymorphism information content(PIC)ranged from 0.115 4 to 0.752 3,and the average was 0.438 9.The 21 pairs of SSR primers could better reflect the genetic diversity information of Staphylea bumalda DC from different sites.The cluster results showed that the genetic similarity coefficient(GS) ranged from 0.58 to 0.93,and the average was 0.78;Staphylea bumalda DC were divided into two broad categories.