SQUAMOSA PROMOTER BINDING PROTEIN-LIKE ( SPL) protein is plant-specific transcription factor,play vital roles in the regulatory responses of plant growth,development and stress.In this study,15 SPL genes were identified from Morus notabilis. MnSPLs transcription factors were clustered into eight groups.Gene structure and motif analysis indicated that most MnSPLs had relatively conserved exonintron arrangements and amino acids motif composition.Several paralogs and orthologs were predicted within the MnSPLs and along with Arabidopsis thaliana and Oryza sativa SPLs.Seven MnSPLs were predicted to be targets of miR156/157.MnSPLs were expressed differentially in various tissues of M.notabilis,six genes of them were highly expressed in five tissue,some genes were mainly highly expressed in winter buds and male flowers.MnSPL genes tissue-specific expression,and played important role in the growth and development of mulberry.