In this study,Lactobacillius was isolated from the feces of healthy adult Chinese pastoral dogs and their prebiotic characteristics were studied.Three strains of Lactobacillus were isolated from the faeces of pastoral dogs using MRS improved medium. The results of morphological observation,physiological and biochemical tests and 16S rRNA gene sequence homology analysis showed that they were identified as L.salivarius,L.johnsonii and L.helveticus, named TCSLl, TCSL2 and TCSL3 respectively.Then acid resistance test,bile salt resistance test,cell adhesion test,bacteriostatic test and oral growth promoting test were carried out in vitro.The results showed that the three strains were tolerant to 0.6% Bile salt,pH value 2.0.The adhesion rate of dog intestinal epithelial cells was more than 65%,and the supernatant had inhibitory effect on dog pathogenic E.coli.There was no diarrhea and death in mice fed for 28 days,TCSL1 and TCSL2 could significantly increase the body weight of mice (P<0.05).Three strains of Lactobacillus isolated from the feces of scattered pastoral dogs have probiotic characteristics,which can be used as candidate strains for developing probiotic preparations for dogs.