In order to investigate the effect of polymorphism of ITPR1 gene RYDR-ITPR domain on eggshell quality in Changshun green-eggshell chicken of Guizhou Province. One pair of primers were designed using online Primer premier 3.0 software to amplify the ITPR1 gene RYDR-ITPR domain.Single nucleotide polymorphism(SNP)was excavated by bidirectional direct sequencing and sequence comparison method.SPSS 18.0 software was used to analyze the influence of SNP sites on the eggshell quality of Changshun green-eggshell chicken.The results showed that three SNP sites were found in the 17th—19th introns of ITPR1 gene,which were g.18853584 G>T and g.18853600 A>G on the 18th intron,and g.18853848 C>T on the 19th exon.The three SNP sites were all moderately polymorphic and did not deviate from the Hardy-Weinberg equilibrium (P>0.05).Four haplotypes and seven diplotypes were detected at three SNP sites.The results of correlation analysis showed that the mutation g. 18853848 C>Ton the 19th exon had a significant effect on eggshell thickness, and TT and CC genotypes were significantly higher than genotype TC (P<0.05).The shell thickness and shell strength of diplotype H3H3 were the highest.To sum up,the mutation g.18853848 C>T in the RYDR-ITPR domain of the ITPR1 gene in Changshun green-eggshell chicken may be a marker site that affects the thickness of eggshell,and diplotype H3H3 may be a favorable type for thickness and strength of eggshell.