In order to understand the prevalence and molecular genetic characteristics of goose parvovirus(GPV) in Hebei Province,in this experiment,virus isolation,pathogenicity and VP gene characteristics were studied using clinically ill Chengde white goose. The results showed that a GPV strain was isolated from the disease material.Artificially infected 6-day-old goslings died at 96 h after infection,and clinical symptoms and necropsy changes were similar to those of the diseased goose.The sequence of the VP gene was spliced,and sequence with length of 2 475 bp was obtained,including VP1,VP2,VP3 three open reading frames.According to genetic evolution analysis and homology analysis, it was found that the isolated GPV HB strain belonged to the Ⅰb Asian group in the genetic evolution tree.The similarity of VP gene between HB strain and other GPV strains was 96.6%—99.7%,and the amino acid similarity with SHFX1201 and Y strains was the highest (99.7%).The above results indicate that, the difference between the HB strain and the domestically isolated GPV strains is small,and the viewpoint that the GPV has only one serotype is confirmed in molecular biology.