A total of 10 strains of Auricularia cornea Ehrenb were used as materials to identify and analyze genetic diversity by RAPD and ISSR.The results showed that the genetic diversity and diversity analysis of the tested strains showed that the genetic diversity was abundant.The RAPD molecular marker had a polymorphic band ratio of 100% and the ISSR molecular marker had a polymorphic band ratio of 93.75%.The combination of RAPD and ISSR was more accurate than one molecular marker alone to identify Auricularia cornea Ehrenb.It could be seen from the genetic clustering map that the difference coefficients between the tested strains ranged from 0.13 to 0.87,and the genetic diversity was abundant.When the difference coefficient D=0.60,all the tested strains were divided into 5 groups,including 2 composite groups and 3 single group. Strains 43 and 43-28 were one population, and the difference distance was 0.48,which indicated that the genetic distance between the two strains was close.The five strains Daguang,Baibeimaomuer,781,Huangbeimuer and Taier 134 were another large population with difference coefficient of 0. 40 and close genetic distance.Baibeimaomuer might be a strain with confused names.Chuanmao 10,99 Feng and 43-1 were three separate populations.The tested strains had abundant genetic diversity,and the genetic distance between three single populations and two complex populations was relatively long,so they could be used as alternative strains for breeding parents.